We narrowed to 15,384 results for: pET

Showing: 9081 - 9100 of 15384 results

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pX330_rActb sgRNA / hSpCas9

Plasmid

#172833

Purpose

Mammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_Tuba1b sgRNA / hSpCas9

Plasmid

#172835

Purpose

Mammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_rSyn1 sgRNA / hSpCas9

Plasmid

#172840

Purpose

Mammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pVRa11_987

Plasmid

#49661

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF11_987

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0001

Plasmid

#42241

Purpose

gRNA targeted to zebrafish gene apoea

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-apoea (apoea Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO/SH/GW-Mst1

Plasmid

#84299

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst1 (STK4 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only
pJT45_GalL_nCDA1-BE3

Plasmid

#145038

Purpose

Expresses nCDA1-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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WPXL LEFTY2 gRNA

Plasmid

#101924

Purpose

LEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

LEFTY2 enhancer targeting gRNA

Use

Lentiviral

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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mADAM8 deltaMP in pQCXIP cFLAG

Plasmid

#19149

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM8 (Adam8 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 228-397

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

Plasmid

#61792

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pVRa15_524

Plasmid

#49668

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF15_524

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg2 (CG510)

Plasmid

#139456

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0002

Plasmid

#42242

Purpose

gRNA targeted to zebrafish gene drd3

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-drd3 (drd3 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

T7

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
TAL3071

Plasmid

#41245

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ets1a-Right (ets1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
TAL3070

Plasmid

#41244

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-ets1a-Left (ets1 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pVRa17_1691

Plasmid

#49672

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF17_1691

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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TAL3403

Plasmid

#47122

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-apoeb-right (apoeb Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3402

Plasmid

#47121

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-apoeb-left (apoeb Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only