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pb-CAGGS-dCas9-blasto

Plasmid

#218555

Purpose

Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

dCas9

Use

CRISPR; Piggybac transposition

Tags

2xSV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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PB-PEmax NG

Plasmid

#187894

Purpose

All-in-one prime editor piggyBac transposon with PEmax, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2-Cb5

Plasmid

#18000

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

ER targeted Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal targeting sequence replac…

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
GFP-Bcl2-Maob

Plasmid

#18001

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Mitochondria-targeted Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Bcl-2's C terminal replaced with mitochondri…

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only
pWZ852

Plasmid

#208114

Purpose

mSwAP-In marker cassette 1 with mScarlet-HygTK

Depositor

Jef Boeke

Article

Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

Insert

mScarlet-HygTK

Tags

T2A

Expression

Bacterial and Mammalian

Promoter

EF1alpha

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS3/myc-His

Plasmid

#127487

Purpose

Expresses myc-tagged NDUFS3 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS3 (NDUFS3 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSecTaq2-hProS-Myc-6xHis

Plasmid

#226524

Purpose

Expresses TAM Receptor Ligand human PROS1

Depositor

Raymond Birge, Sergei Kotenko

Article

Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

Insert

Human PROS1 (PROS1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-TAZ-S89A

Plasmid

#213588

Purpose

Doxycycline inducible expression of TAZ-S89A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

TAZ (WWTR1 Human)

Use

Lentiviral

Tags

2xHA

Expression

Mammalian

Mutation

S89A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
AAV-TRE-NS1-dTomato

Plasmid

#175278

Purpose

AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

NS1; dTomato (POLY Yellow fever virus strain 17D)

Use

AAV

Tags

dTomato (from bidirectional promoter)

Promoter

bidirectional TRE promoter

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-NS1

Plasmid

#175273

Purpose

AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)

Use

AAV

Promoter

Synapsin

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 S192A

Plasmid

#69820

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Tags

V5

Expression

Mammalian

Mutation

S192A

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-XRCC1-EGFP-Hygro

Plasmid

#176062

Purpose

EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.V5.VSVg_NGFR

Plasmid

#158244

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

HA.V5.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2/myc-His

Plasmid

#127486

Purpose

Expresses myc-tagged NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pThermoCas9i_ctrl

Plasmid

#100982

Purpose

Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Mutation

changed aspartate 8 to alanine and histidine 582 …

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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