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pcDNA3-HA-MKRN1

Plasmid

#78745

Purpose

To overexpress MKRN1 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSH1-wSQR

Plasmid

#256133

Purpose

Inducible expression of sulfide:quinone oxidoreductase from Wolinella succinogenes

Depositor

Benjamin Woolston

Publication

Fernez et al ACS Synth Biol. 2025 Jun 20;14(6):2198-2

Insert

sqr

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPRv2 sgSLC38A2#1

Plasmid

#237230

Purpose

Plasmid for SNAT2 (SLC38A2) CRISPR knockout in human cell lines.

Depositor

Seth Parker

Publication

Koe et al Am J Physiol Cell Physiol. 2026 Jun 29.

Insert

Inserted sgRNA targetting SLC38A2 (SLC38A2 Mouse, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 1, 2026

Availability

Academic Institutions and Nonprofits only

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eGFP-MKRN2-dRING-pcDNA4/TO

Plasmid

#257246

Purpose

Expresses eGFP-MKRN2 with mutated RING domain under tetracycline inducible promoter. All cysteine and histidine residues in the RING domain were mutated to alanine.

Depositor

Eran Hornstein

Publication

Amzallag et al EMBO Rep. 2026 Jun 15. doi: 10.1038/s443

Insert

eGFP-MKRN2--dRING (MKRN2 Human)

Tags

eGFP

Expression

Mammalian

Available since

June 24, 2026

Availability

Academic Institutions and Nonprofits only

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pMF2-rSQR

Plasmid

#256132

Purpose

Inducible expression of sulfide:quinone oxidoreductase from Rhodobacter capsulatus

Depositor

Benjamin Woolston

Publication

Fernez et al ACS Synth Biol. 2025 Jun 20;14(6):2198-2

Insert

sqr

Expression

Bacterial

Mutation

Codon harmonized for E. coli expression

Promoter

pBAD

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pIC-RFP-2

Plasmid

#254929

Purpose

All-in-one plasmid developed for inducible CRISPRi-assisted genome editing in Streptomyces

Depositor

Gilles van Wezel

Publication

Bai et al Nucleic Acids Res. 2026 Jun 8;54(11):gka

Insert

dCas9

Use

CRISPR and Synthetic Biology

Available since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA4173 [CMV-TRNAU1AP(Δ184-278)-FLAG]

Plasmid

#254880

Purpose

Expresses CMV-driven C-terminal FLAG-tagged TRNAU1AP (Δ184-278)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Amino acid 184-278 truncated TRNAU1AP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA4176 [CMV-TRNAU1AP(Δ248-278)-FLAG]

Plasmid

#254881

Purpose

Expresses CMV-driven C-terminal FLAG-tagged TRNAU1AP (Δ248-278)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Amino acid 248-278 truncated TRNAU1AP

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-B_sgRNA_WDR61 (pAVA3321)

Plasmid

#254886

Purpose

Lentiviral vector expressing Cas9-P2A-Blasticidin and an sgRNA targeting WDR61

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

Sinlge sgRNA targeting WDR61

Use

CRISPR and Lentiviral

Promoter

U6

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA3932 [TRNAU1AP minigene: exon3-intron3 including poison exon-exon4]

Plasmid

#254878

Purpose

Expresses EFS-driven TRNAU1AP minigene (exon3--poison exon-containing intron3-exon4)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

TRNAU1AP minigene (exon3-poison exon-containing intron3-exon4)

Expression

Mammalian

Promoter

EFS

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAVA3936 [PRPF39 minigene: exon8-intron8 including poison exon-exon9]

Plasmid

#254879

Purpose

Expresses EFS-driven PRPF39 minigene (exon8-poison exon-containing intron8-exon9)

Depositor

Andrei Alexandrov

Publication

Panah et al Nat Commun. 2026 Jun 11. doi: 10.1038/s4

Insert

PRPF39 minigene (exon8-poison exon-containing intton8-exon9)

Expression

Mammalian

Promoter

EFS

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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NCMV HPV101 E7

Plasmid

#253678

Purpose

Expresses N-Flag/HA HPV101 E7

Depositor

Karl Munger

Publication

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Tags

Flag/HA

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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CMV-C 101 E7

Plasmid

#253675

Purpose

Expresses C-Flag/HA HPV101 E7

Depositor

Karl Munger

Publication

Gelbard et al Tumour Virus Res. 2025 Jun;19:200300. do

Insert

HPV101 E7

Tags

Flag/HA

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Lenti ccdB Dang sgRNA Hyg sfGFP

Plasmid

#235047

Purpose

Lentiviral sgRNA expression with optimized sgRNA scaffold vector with hygromycin and superfolder GFP. Also contains ccdB cassette for library cloning.

Depositor

Justin Pritchard

Publication

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ432:SSX1p-mK2-Ex1-(5'D)-ID-(linker1)-pA

Plasmid

#251335

Purpose

Expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ433:H2A1p-(linker2)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA

Plasmid

#251336

Purpose

Expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ500:G5p-mKate2-pA

Plasmid

#251337

Purpose

Expression of mKate2 under G5p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mKate2

Expression

Mammalian

Promoter

G5p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ501:G5p-IL15-pA

Plasmid

#251338

Purpose

Expression of IL-15 under G5p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

IL-15

Expression

Mammalian

Promoter

G5p

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ330:CMVp-mK-Ex1-miRS3-mK-Ex2-PEST-miRS1-BSs-miRS2-BSs-pA

Plasmid

#251325

Purpose

Expression of mKate2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

mKate2

Expression

Mammalian

Promoter

CMVp

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ334:CMVp-BD6-AS-(3'SE)-EC-Ex2-miRS2-BSs-pA

Plasmid

#251326

Purpose

Expression of ECFP-Ex2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Publication

Roas et al Nat Commun. 2026 Jun 16. doi: 10.1038/s4

Insert

EC-Ex2

Expression

Mammalian

Promoter

CMVp

Available since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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