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Plasmid#177027PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsert7-deoxyloganetic acid glucosyl transferase; UGT709C2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0060
Plasmid#177024PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertiridoid synthase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0059
Plasmid#177023PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsert8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0063
Plasmid#177021PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsertgeraniol synthase
UseSynthetic BiologyTagsExpressionMutationtransit peptide removedPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBHRSF228
Plasmid#135007PurposeMusF2 prenyltransferases from Nostoc sp. UHCC 0398DepositorInsertMusF2 prenyltransferase
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E18: attB4-polyhedrinp>-attB5
Plasmid#162977PurposeGateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoterDepositorInsertAcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E21: attB4-FerHp>-attB5
Plasmid#162978PurposeGateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoterDepositorInserthuman Ferritin heavy chain promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r
Plasmid#162936PurposeGateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoterDepositorInsertTriple FLAG epitope tag fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E01: attB5r-meGFP-nostop-attB1r
Plasmid#162940PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r
Plasmid#162937PurposeGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoterDepositorInsertHis6 fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E11: attB5r-RU5u-His6-nostop-attB1r
Plasmid#162953PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E25: attB4-mPol2p>-attB5
Plasmid#162981PurposeGateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Plasmid#162956PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHis6-Halotag fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r
Plasmid#162935PurposeGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoterDepositorInsertMaltose-binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C453-E09: attB4-Grp78p>-attB5
Plasmid#162974PurposeGateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoterDepositorInserthuman Glucose response protein 78 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-2
Plasmid#163128PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E09: attB5r-RU5u-FLAG-nostop-attB1r
Plasmid#162951PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertFLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E59: attB5r-RU5u-2xHA-nostop-attB1r
Plasmid#162966PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteinsDepositorInsertDual HA epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E42: attB5r-RU5u-Strep2-nostop-attB1r
Plasmid#162961PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteinsDepositorInsertStrep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E26: attB5r-RU5u-myc-nostop-attB1r
Plasmid#162960PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteinsDepositorInsertMyc epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only