We narrowed to 19,197 results for: gateway
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TFORF3482
Plasmid#144958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-SB
Plasmid#177936PurposeExpresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.DepositorTypeEmpty backboneUseCRISPR; TransposonExpressionMammalianPromoterU6Available SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAPDH
Plasmid#155622PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-EGFP-P2A-Puro
Plasmid#160226PurposeExpresses EGFP in mammalian cellsDepositorInsertEGFP-P2A-PuroR
UseRetroviralExpressionBacterial and MammalianPromoterLTRAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xflag-APEX2-LRRK2
Plasmid#164622PurposeExpresses LRRK2 tagged with APEX2 and 3xFLAGDepositorAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD WT
Plasmid#169713PurposeBacterial expression of human hnRNPA1-A Low complexity domain including amino acids 186-320DepositorInserthnRNPA1 (HNRNPA1 Human)
Tags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
dTAG NLRP1
Plasmid#166825PurposedTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradationDepositorAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163072PurposeEntry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseGateway CloningAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)
Plasmid#163075PurposeEntry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)DepositorInsertroGFP2-Orp1
UseGateway CloningAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS019: ClopHensorN chloride sensor (cytosolic)
Plasmid#163048PurposeClopHensorN chloride sensor (cytosolic)DepositorInsertClopHensorN
UseLentiviralTagstdTomatoExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2475 - pEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
Plasmid#159813PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | mosase(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX302 OASL-V5 puro
Plasmid#158643PurposeLentiviral expression vector for constitutive expression of V5-tagged OASL.DepositorAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
ExpressionMammalianAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFP-TTP
Plasmid#141190PurposeExpresses GFP-TTP in mammalian cells, can be used to make inducible cell lineDepositorAvailable SinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3TS_Transposase
Plasmid#109768PurposeCodon optimised transposase sequence in an optimal vector for in vitro transcription. Contains the T3 promoter and synthetic polyA tract.DepositorInsertTol2 Transposase
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA
Plasmid#134923PurposeComponents for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.DepositorInsertCascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
ExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:tdTomato
Plasmid#135214PurposeRed tagged macrophagesDepositorInsertmfap4 promoter and tdTomato
UseZebrafish expressionAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
A3A-PBE
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV 3xFLAG-LC3C-GFP
Plasmid#123110PurposeExpresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3CDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
Tags3xFLAG and EGFPExpressionMammalianPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only
