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pcDNA3/Flag-METTL14

Plasmid

#53740

Purpose

For expression of METTL14 (methyltransferase-like 14) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 14 (METTL14 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin-Flpo

Plasmid

#60663

Purpose

Expresses pAAV-hSynapsin-Flpo

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Flpo

Use

AAV

Promoter

hSynapsin1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDULE-ABK

Plasmid

#49086

Purpose

A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

Depositor

Peter Schultz

Article

Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

Inserts

tRNA synthetase
tRNA for a photocrosslinking amino acid

Expression

Bacterial

Mutation

L274M, C313A, Y349F

Promoter

GlnRS and lpp

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-mScarlet

Plasmid

#207678

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDV05.0_pEF1a-iRFP720

Plasmid

#200407

Purpose

Expresses iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-miniAAVR-FLAG

Plasmid

#166717

Purpose

Vector expressing a minimal AAVR (KIAA0319L) construct PKD1-3 with a C-terminal Flag tag

Depositor

Jan Carette

Article

Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

Insert

miniAAVR-FLAG

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Only expressing PKD1-3 of ectodomain

Promoter

CMV

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189923

Purpose

AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

nSaCas9 KKH, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP_WT-talin1 (1-2541)

Plasmid

#166112

Purpose

For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

WT-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-mCherry

Plasmid

#180646

Purpose

Lentiviral expression vector for mCherry. Dox-inducible. Internal ID: WISP20-61.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1152

Plasmid

#142739

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYOCD (MYOCD Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9-10xSunTag(22aa)

Plasmid

#215745

Purpose

Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

dCas9-10xSunTag

Expression

Mammalian

Promoter

chicken beta-actin promoter

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PE6f

Plasmid

#207856

Purpose

Mammalian expression of PE6f prime editor

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

PE6f

Tags

SV40 bpNLS and SV40 bpNLS, c-Myc NLS

Expression

Mammalian

Mutation

Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise2-IL-1beta-mNeonGreen

Plasmid

#166783

Purpose

FRET analysis (pro-IL-1beta cleavage) in living cells

Depositor

Hao Wu

Article

Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

Insert

mTurquoise2-IL-1b-mNeongreen (IL1B Human)

Use

Lentiviral

Tags

mNeonGreen and mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-myc

Plasmid

#20211

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pJeM1

Plasmid

#135088

Purpose

rhamnose-inducible expression vector for replication in E. coli and P. putida

Depositor

Josef Altenbuchner

Article

Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

Insert

rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Cortactin-pmCherryC1

Plasmid

#27676

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cortactin (Cttn Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pDONR223_PCCB_WT_V5

Plasmid

#82938

Purpose

Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PCCB (PCCB Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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