162,094 results

1. pCDFDuet-MKK6-EE p38alpha

   Plasmid

   #82719

   Purpose

   Expresses constitutively active human MKK6 and murine p38 alpha.

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Inserts

   MKK6 (MAP2K6 Human)
   p38alpha (Mapk14 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Ser207 to Glu, Thr211 to Glu

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pCXLE-gw

   Plasmid

   #37626

   Purpose

   Non-integrating (episomal) expression vector with Gateway cassette

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti SYN-FLAG-dCas9-VPR

   Plasmid

   #114196

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SYN

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKV016

   Plasmid

   #64767

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ubc.Luc.IRES.Puro

   Plasmid

   #33307

   Depositor

   Linzhao Cheng

   Article

   Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lamin A-mRFP

   Plasmid

   #124268

   Purpose

   Wild-type Lamin A, C-terminally tagged with mRFP

   Depositor

   Eric Schirmer

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tet-O-FUW-EGR1-P2A-mCherry

   Plasmid

   #203856

   Purpose

   Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   EGR1 (EGR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-GGamma2-GFP2

   Plasmid

   #166774

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma2-GFP2 (GNG2 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRK892

    Plasmid

    #71803

    Purpose

    Expression of mutated L. gasseri gusA from strong Ppgm promoter

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

    Insert

    gusA3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D573A

    Promoter

    Ppgm

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. CMV-LUC2CP/ARE

    Plasmid

    #62857

    Purpose

    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    destabilizing sequences (DS) added to the C termi…

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLAMP1-miRFP670nano3

    Plasmid

    #184669

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    LAMP1-miRFP670nano3
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV5 human p27

    Plasmid

    #14049

    Depositor

    Joan Massague

    Article

    Luo et al Nature. 1995 May 11. 375(6527):159-61.

    Insert

    p27 (CDKN1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEMHE-mCherry-mTrim21

    Plasmid

    #105522

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACBSR-hyg

    Plasmid

    #87830

    Purpose

    Expresses lambda-red system with hyg selection marker

    Depositor

    Pep Charusanti

    Article

    Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

    Insert

    hyg
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    None

    Promoter

    bla

    Available since

    Aug. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR-A-HYG

    Plasmid

    #29635

    Depositor

    David_L Andrews, Scott Gold

    Article

    Garcia-Pedrajas et al Fungal Genet Biol. 2008 Apr . 45(4):379-

    Type

    Empty backbone

    Use

    Gateway donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pET-28a-hPKM1

    Plasmid

    #44241

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M1 (PKM Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH2B-LSSmOrange

    Plasmid

    #37133

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

    Insert

    LSSmOrange
    

    Use

    Tags

    histone 2B

    Expression

    Mammalian

    Mutation

    A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

    Promoter

    CMV

    Available since

    June 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge