164,547 results

1. pJBL3907

   Plasmid

   #193140

   Purpose

   Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

   Depositor

   Julius Lucks

   Article

   Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

   Insert

   Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL6090

   Plasmid

   #193142

   Purpose

   Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporter

   Depositor

   Julius Lucks

   Article

   Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

   Insert

   Cbe ZTP Riboswitch sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Deleted A26 to disable ligand-binding

   Promoter

   J23119

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Portal_PhiV1

   Plasmid

   #188811

   Purpose

   Portal protein from phage PhiV1

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Portal
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Terminase_ST32

   Plasmid

   #188850

   Purpose

   Large terminase from phage ST32

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   Terminase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EpAvs1

   Plasmid

   #188788

   Purpose

   Avs1 from Erwinia piriflorinigrans CFBP5888

   Depositor

   Feng Zhang

   Article

   Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

   Insert

   EpAvs1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   bla

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpPLK1-GFP

   Plasmid

   #185573

   Purpose

   to see protein dynamics

   Depositor

   Mamiko Yajima

   Article

   Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

   Insert

   PLK1
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK19 pCAS-Tyr-[gRNA: 2=FF21] (SplitHygR, AmpR)

   Plasmid

   #179003

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PPDK

   Plasmid

   #64037

   Purpose

   Expression of Pyruvate phosphate dikinase (PPDK) in E. coli

   Depositor

   Vern Schramm

   Article

   Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do

   Insert

   pyruvate phosphate dikinase
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-SADB19G

   Plasmid

   #32633

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   SADB19G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9/pTREX-n

    Plasmid

    #68708

    Purpose

    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    

    Use

    CRISPR

    Tags

    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …

    Expression

    Mutation

    C4239T mutation that eliminates a BamHI restricti…

    Promoter

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-MBP-BrCas12b-RFND

    Plasmid

    #195339

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis and 6xHis, MBP, TEV cleavage site

    Expression

    Bacterial

    Mutation

    R160E, F208W, N524V, D868V

    Promoter

    T7

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV hSyn FLEx-FRT hM4Di-mCherry

    Plasmid

    #161576

    Purpose

    Expression of hM4Di-mCherry in a Flp-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ULK2

    Plasmid

    #97075

    Purpose

    Expresses ULK2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    ULK2 (ULK2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Tsg101

    Plasmid

    #21505

    Depositor

    James Hurley

    Article

    Lee et al Science. 2008 Oct 24. 322(5901):576-80.

    Insert

    Tsg101 (TSG101 Human)
    

    Use

    Tags

    Cherry*

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. spNW30

    Plasmid

    #173485

    Purpose

    Expresses spNW30 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW30
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pemiRFP670-tubulin

    Plasmid

    #136573

    Purpose

    Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    emiRFP670-tubulin
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSXneo 135(T2AG3)

    Plasmid

    #12402

    Depositor

    Titia de Lange

    Article

    Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

    Insert

    135(T2AG3)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-puro-Bcl-XL

    Plasmid

    #46972

    Purpose

    lentiviral expression of Bcl-XL

    Depositor

    Jialiang Wang

    Article

    Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPAGFP-N1

    Plasmid

    #11909

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

    Has Service

    Cloning Grade DNA

    Insert

    pPAGFP-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    These plasmids are essentially the same as the Cl…

    Promoter

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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