166,276 results

1. mEos4b-TOMM20-N-10

   Plasmid

   #57518

   Purpose

   Localization: Mitochondrial Membrane, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson, Loren Looger

   Insert

   TOMM20 (TOMM20 Human)
   

   Tags

   mEos4b

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCW57.1-FOXM1b

   Plasmid

   #68811

   Purpose

   Doxycycline inducible lentiviral vector for FOXM1b expression in mammalian cells

   Depositor

   Adam Karpf

   Article

   Barger et al Oncotarget. 2015 Jul 16.

   Insert

   FOXM1b (FOXM1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-mCherry-Nanobody (LaM-2)

   Plasmid

   #162276

   Purpose

   Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

   Insert

   mCherry-Nanobody (LaM-2)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

   Plasmid

   #219548

   Purpose

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

   Insert

   NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
   

   Expression

   Mammalian

   Mutation

   The (SAGG)x4 linker between GAL4(1-65) and the PC…

   Promoter

   CMVd1

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM200

   Plasmid

   #13079

   Depositor

   Kenan Murphy

   Article

   Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

   Insert

   Ptac-gam-bet-exo operon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sfp pet29b C-terminal His Tag

   Plasmid

   #75015

   Purpose

   Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

   Depositor

   Michael Burkart

   Article

   Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

   Insert

   Sfp
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

   Plasmid

   #188384

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. tFucci(CA)5pPBbsr2

   Plasmid

   #197351

   Purpose

   Encoding tFucci(CA)5.

   Depositor

   Michiyuki Matsuda

   Article

   Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

   Insert

   tFucci(CA)5
   

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-PD-1-SNAP

   Plasmid

   #223576

   Purpose

   Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAT201

    Plasmid

    #134878

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-KIF2C

    Plasmid

    #205996

    Purpose

    inducible expression of HA-KIF2C

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    KIF2c (KIF2C Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    pTight

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZA69

    Plasmid

    #174782

    Purpose

    RNAi

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    Intron-containing hairpin RNA (ihpRNA)-NbZAR1
    

    Use

    RNAi

    Mutation

    WT

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-uvsX-H6

    Plasmid

    #163913

    Purpose

    Expresses uvsX for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsX
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-PGK-ABI-dCas9-P2A-mcherry

    Plasmid

    #121513

    Purpose

    expression of AB1-fused-dcas9 plasmids

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    AB1 and P2A-mcherry

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-A3-HA-ubiquitin

    Plasmid

    #220145

    Purpose

    Expresses A3-Ub for E3 substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    A3-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Article

    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Promoter

    EF1alpha and mU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2545

    Plasmid

    #144038

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CEBPB (CEBPB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMP71-Flt3L

    Plasmid

    #174600

    Purpose

    Secretes the extracellular domain of murine Flt3L

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    secretes extracellular domain of mouse Flt3-L
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKrox24(2xD-E_inD)Luc

    Plasmid

    #200109

    Purpose

    Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element in front of EGR1 promoter…

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-mcBEST

    Plasmid

    #209416

    Purpose

    Plasmid for multiplexed cytosine base editing in streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

    Inserts

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    base editing cassette: PtipA ; sgRNAs: PkasO* an…

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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