|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74289||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
Currently unavailable outside the U.S.
This material is available to academics and nonprofits only.
Backbone manufacturerK. Deisseroth lab (Stanford)
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameH2B-GFP and oG (optimized Glycoprotein)
Speciesglycoprotein for rabies virus SAD B19
- Promoter Ef1a
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH was a gift from Edward Callaway (Addgene plasmid # 74289 ; http://n2t.net/addgene:74289 ; RRID:Addgene_74289)
For your References section:Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies Virus Glycoproteins. Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM. Cell Reports 2016 10.1016/j.celrep.2016.03.067