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pX330-PITCh-ARL13B

Plasmid

#227276

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of ARL13B (ARL13B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJP_Azul01

Plasmid

#219670

Purpose

Blue light inducible system

Depositor

Yolanda Schaerli

Article

Park et al Nat Commun. 2024 Aug 23;15(1):7284. doi:

Inserts

similar to T7 RNA polymerase (core)
similar to YF1-fixJ

Use

Synthetic Biology

Promoter

LacIq35 and pJ23105

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pINSPECT_SigP:NLuc

Plasmid

#197263

Purpose

Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-HsSAS6

Plasmid

#136790

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pNR2F1.1.0-gDNA

Plasmid

#112484

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1

Depositor

Kevin White

Insert

NR2F1 (NR2F1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSin Capsid

Plasmid

#127693

Purpose

Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

Depositor

Justin English, Bryan Roth

Article

English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

Insert

Sindbis Capsid

Use

Synthetic Biology

Available Since

July 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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LCV2 IRF3 KO

Plasmid

#217446

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting IRF3 (IRF3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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ptwB

Plasmid

#48735

Purpose

Tandem dual tomato plasmid

Depositor

John Schiller, Roger Tsien

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

Tandem dual tomato

Expression

Mammalian

Promoter

EF1a

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-UTY-F

Plasmid

#40617

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTY (UTY Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1

Available Since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
pET29b_mNeonGreen

Plasmid

#209425

Purpose

Express mNeonGreen in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

mNeonGreen

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pMHG-W57* reporter

Plasmid

#72850

Purpose

GFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.

Depositor

Daniel Liebler

Article

Halvey et al FEBS Open Bio. 2012 Dec;2:56-59. Epub 20

Insert

GFP

Expression

Mammalian

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLV6-mPER2-luc

Plasmid

#212035

Purpose

Expression of murine Per2-luciferase in mammalian cells

Depositor

David Hazlerigg

Article

Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

Insert

mPer2-luciferase (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-BRCA2

Plasmid

#99395

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged BRCA2

Depositor

Junjie Chen, Michael Huen

Article

Sy et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

BRCA2 (BRCA2 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-STING-sh5

Plasmid

#127647

Purpose

Knock-down of human STING

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

STING shRNA (STING1 Human)

Use

Lentiviral

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-TEAD1

Plasmid

#33109

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD1 (TEAD1 )

Tags

Myc

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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UAS:mCherry

Plasmid

#170994

Purpose

UAS:GAL4 repeat-containing promoter drives simple mCherry reporter gene. Blasticidin selectable.

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

UAS:mCherry

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

Synthetic 14x UAS:Gal4

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET GFP LIC cloning vector (u-msfGFP)

Plasmid

#29772

Depositor

Scott Gradia

Type

Empty backbone

Tags

SiriusGFP

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pROSA26-1

Plasmid

#21714

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe_Hyg_cOVA_T2A_mStrawberry

Plasmid

#161737

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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