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pDONR223-PRKAR2A

Plasmid

#23789

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR2A (PRKAR2A Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Use

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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SIRT1.1

Plasmid

#13735

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

SIRT1 (SIRT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-beta-HA-BRCA2

Plasmid

#133553

Purpose

Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

Depositor

David Livingston

Article

Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Insert

BRCA2 (BRCA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCMV-Blast

Plasmid

#125133

Purpose

Lentiviral empty backbone control vector with CMV promoter and Blasticidin resistance gene

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMRXIP GFP-Stx17 WT

Plasmid

#45909

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pBT375

Plasmid

#125616

Purpose

Expresses evoAPOBEC1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGmRuby2smFPV5

Plasmid

#71817

Purpose

encodes multiple copies of an epitope tag V5( not detected by anti GFP ab)

Depositor

Loren Looger

Insert

mRuby2_smFP_V5

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
TFORF2119

Plasmid

#141930

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HOPX (HOPX Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL MND WASp

Plasmid

#36248

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

WASp (WAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

MND

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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lenti-NLS-mTagBFP2

Plasmid

#216128

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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GSK3beta (1015)

Plasmid

#49491

Purpose

expresses human Gsk3beta

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta (GSK3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pSPObooster

Plasmid

#216160

Purpose

Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

Depositor

Grant Brown

Article

Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

Inserts

MKT1(30G) (MKT1 Budding Yeast)
RME1(ins-308A) (RME1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

MKT1 gene containing an aspartic acid to glycine …

Promoter

endogenous promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChRmine-eYFP-WPRE

Plasmid

#130996

Purpose

Double floxed ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Expression

Mutation

Promoter

Ef1a

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cas9-T2A-TdT

Plasmid

#126424

Purpose

Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

Cas9-T2A-TdT (DNTT Human, Streptococcus pyogenes)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTH101

Plasmid

#84459

Purpose

pJB38-NWMN29-30 + SarA_P1-DsRed-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-DsRED from pTH1

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-COX4-dAPEX2

Plasmid

#117177

Purpose

Plasmid name in publication: pAAV-DIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

COX4-dAPEX2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-CreN-InteinN

Plasmid

#187614

Purpose

AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

CreN-InteinN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1367

Plasmid

#143492

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

RREB1 (RREB1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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