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pFUW H2B-mTagBFP2-P2A-PuroR

Plasmid

#239552

Purpose

Expression and nuclear localization of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO Cre-2A-GFP

Plasmid

#68450

Purpose

TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cre-2A-GFP

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-sesRNA-GFP-WPRE-pA

Plasmid

#192065

Purpose

Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Cheta

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1688

Plasmid

#144371

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HNF1A (HNF1A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMGIB-3xFLAG-dyskerin

Plasmid

#53607

Purpose

Retroviral expression of human dyskerin

Depositor

Steven Artandi

Article

Venteicher et al Science. 2009 Jan 30;323(5914):644-8. do

Insert

dyskerin (DKC1 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-STAT2

Plasmid

#71451

Purpose

3rd generation lentiviral expression of STAT2

Depositor

George Stark

Article

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

STAT2 (STAT2 Human)

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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SIRT5 Flag

Plasmid

#13816

Purpose

Mammalian expression of human SIRT5 with flag tag

Depositor

Eric Verdin

Article

North et al Mol Cell. 2003 Feb . 11(2):437-44.

Insert

SIRT5 (SIRT5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216246

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgAAVS1.5

Use

CRISPR; Human safe harbor locus (a avs1) site-spe…

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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psgRNA

Plasmid

#114005

Purpose

express sgRNA. ColE1, Kan

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Insert

gRNA

Promoter

BBa_J23119

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLgw V5-EcoDam

Plasmid

#59210

Purpose

Mammalian DamID control lentiviral vector for expression of V5-Dam only

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

EcoDam

Use

Lentiviral; Damid

Tags

V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag p38 alpha

Plasmid

#20815

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 alpha (Mapk14 Mouse)

Tags

Flag

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VRQR

Plasmid

#159983

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VRQR

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-VASP-5

Plasmid

#55151

Purpose

Localization: Actin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

VASP

Tags

mCherry

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hDNAJC6-T2A-copGFP

Plasmid

#170443

Purpose

Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human DNAJC6 (DNAJC6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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moxGFP

Plasmid

#68070

Purpose

C-terminal fusion to moxGFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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mCardinal-N1

Plasmid

#54590

Purpose

Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Type

Empty backbone

Tags

mCardinal

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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