Purposeexpresses CD4 with C-terminal FLAG tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51604||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5300
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1300
Entrez GeneCD4 (a.k.a. CD4mut)
- Promoter CMV
/ Fusion Proteins
- FLAG (C terminal on insert)
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer gagacacggagagggtcttc
- 3′ sequencing primer CATTCTAGTTGTGGTTTGTCC (Common Sequencing Primers)
Terms and Licenses
Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The CD4 insert in this plasmid contains N64I and K385E mutations when compared to GenBank reference sequence NP_038688.2.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-CD4-FLAG was a gift from Suzanne Paradis (Addgene plasmid # 51604 ; http://n2t.net/addgene:51604 ; RRID:Addgene_51604)
For your References section:Sema4D localizes to synapses and regulates GABAergic synapse development as a membrane-bound molecule in the mammalian hippocampus. Raissi AJ, Staudenmaier EK, David S, Hu L, Paradis S. Mol Cell Neurosci. 2013 Nov;57:23-32. doi: 10.1016/j.mcn.2013.08.004. Epub 2013 Sep 10. 10.1016/j.mcn.2013.08.004 PubMed 24036351