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pMX hCD4
(Plasmid #14614)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14614 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMX
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Entrez Gene
    CD4 (a.k.a. CD4mut)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBMN5'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX hCD4 was a gift from Dan Littman (Addgene plasmid # 14614 ; http://n2t.net/addgene:14614 ; RRID:Addgene_14614)
  • For your References section:

    The envelope glycoprotein of the human immunodeficiency virus binds to the immunoglobulin-like domain of CD4. Landau NR, Warton M, Littman DR. Nature. 1988 Jul 14. 334(6178):159-62. 10.1038/334159a0 PubMed 3260352