176,531 results

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CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Publication

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HA-14-3-3 epsilon

Plasmid

#116886

Purpose

Expresses HA-tagged human 14-3-3 epsilon in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Publication

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 epsilon (YWHAE Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMx-puro-MGT

Plasmid

#111809

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Publication

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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plenti-CMV-mCherry-T2A-GFP

Plasmid

#109427

Purpose

A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

Depositor

Reuben Harris

Publication

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry T2A GFP

Use

Lentiviral

Promoter

CMV

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAV-Syn1-tTA

Plasmid

#104109

Purpose

AAV-mediated expression of tTA under the Syn1 promoter.

Depositor

Takeshi Imai

Publication

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Use

AAV

Promoter

hSyn1

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mKate2-P2A-APEX2-csGBP

Plasmid

#108875

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP (includes red cytoplasmic reporter)

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mKate2-P2A-APEX2-csGBP

Expression

Mammalian

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUS-FusionRed-PixD

Plasmid

#111503

Purpose

For PixELL system

Depositor

Jared Toettcher

Publication

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

PixD (slr1694 )

Use

Lentiviral

Tags

FUS and Fusion Red

Promoter

SFFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.18 CMV-Luc

Plasmid

#100984

Purpose

CMV luciferase reporter - firefly luciferase driven by CMV promoter

Depositor

Lee Helman

Publication

Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

Insert

CMV

Expression

Mammalian

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLVET-HA-K-RasG12V-IRES-GFP

Plasmid

#107140

Purpose

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

Depositor

Aki Manninen

Publication

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

HA-tagged Kras4B(G12V) and GFP (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hEF-1alpha

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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WT Smarca4-V5 IRES-puro

Plasmid

#107058

Purpose

Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

Depositor

Jerry Crabtree, Courtney Hodges

Publication

Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

Insert

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)

Use

Lentiviral

Tags

IRES-puroR and V5

Expression

Mammalian

Mutation

wild-type

Promoter

CAG

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pHIV-H2B-eGFP

Plasmid

#91776

Purpose

Expresses H2B protein tagged with eGFP

Depositor

Maria Pia Cosma

Publication

Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

Insert

Human Histone H2B / eGFP (H2BC11 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMSCV-hygro-STING V155M

Plasmid

#102599

Purpose

Express STING V155M for IFN-beta Luciferase reporter assay.

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

V155M

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTO_CHMP4B_LAP

Plasmid

#101850

Purpose

Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

Depositor

Daniel Gerlich

Publication

Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

Insert

Charged Multivesicular Body Protein 4B (CHMP4B Human)

Use

Gateway cloning, flp-frt recombination, tet-induc…

Tags

Localization and Affinity Purification (LAP) tag

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR_Gal4UAS_Blinatumomab_PGK_mCherry

Plasmid

#85437

Purpose

[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Blinatumomab

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Publication

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAD-GamS

Plasmid

#99246

Purpose

Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

Depositor

Jeff Hasty

Publication

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

gamS (with C-terminal 6xHis tag)

Tags

6xHis tag

Expression

Bacterial

Promoter

T7lac

Available since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-teLuc c-myc

Plasmid

#100026

Purpose

Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

Depositor

Huiwang Ai

Publication

Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

Insert

teLuc, a NanoLuc mutant

Tags

c-myc

Expression

Mammalian

Mutation

D19S, D85N, C164H

Promoter

CMV

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-WT

Plasmid

#101047

Purpose

Expresses HA-tagged human Rab11-WT in mammalian cells

Depositor

Ken-Ichi Takemaru

Publication

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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