162,109 results

1. pcDNA-Malibu-mScarlet

   Plasmid

   #229554

   Purpose

   Fluorescent reporter for malonyl-CoA

   Depositor

   Danielle Schmitt

   Article

   Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

   Insert

   Malibu-mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-Kif2c

   Plasmid

   #208651

   Purpose

   Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

   Depositor

   Samuel Bakhoum

   Article

   Li et al Nature. 2023 Aug;620(7976):1080-1088. do

   Insert

   Kif2c (KIF2C Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-mCherry

   Plasmid

   #108122

   Purpose

   Recruitable fluorophore

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   FKBP1A(isoform a, 3-108):mCherry (FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-C1-mTrim21

   Plasmid

   #105516

   Purpose

   Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRTC2-puro

   Plasmid

   #190567

   Purpose

   Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

   Depositor

   Anthony Furano

   Article

   Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   L1 (L1.3) with reporter cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #161927

   Purpose

   Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   Surface marker and Citrine repression reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJFRC19-13XLexAop2-IVS-myr::GFP

   Plasmid

   #26224

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   myr::GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-DIO-EF1α-mKate2-biPAC

   Plasmid

   #169127

   Purpose

   AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Insert

   mKate2-biPAC
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   codon optimized to mouse

   Promoter

   EF1a

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MscL(G22S)61-cpGFP-ERexp

   Plasmid

   #195311

   Purpose

   Fluorescent reporter/biosensor for membrane tension

   Depositor

   Allen Liu

   Article

   Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

   Insert

   MscL(G22S)61-cpGFP-ERexp
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG_smFP Myc

    Plasmid

    #59757

    Purpose

    encodes multiple copies of an epitope tag Myc

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _Myc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWSK29

    Plasmid

    #172972

    Purpose

    Low copy number cloning vector

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA4.1-Myc-RNF8

    Plasmid

    #221676

    Purpose

    Mammalian expression of Myc-tagged human RNF8

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    RNF8 (RNF8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHaloTag-EGFP

    Plasmid

    #86629

    Purpose

    Expresses HaloTag-eGFP tandem (cytosolic)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST26-NAT1-C-HA

    Plasmid

    #195178

    Purpose

    Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

    Depositor

    Stephanie Roessler

    Insert

    NAT1 (NAT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-CMV-V5-RFP-SLMAP (isoform r)

    Plasmid

    #235685

    Purpose

    Express RFP tagged SLMAP gene in mammalian cells using the CMV promoter

    Depositor

    Duojia Pan

    Article

    Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

    Insert

    SLMAP (SLMAP Human)
    

    Use

    Lentiviral

    Tags

    V5-RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSyn.flex.H2B.GFP

    Plasmid

    #170368

    Purpose

    Cre-inducible AAV vector expressing nuclear GFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    flex H2B GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCAR_sgRNA_puro-mKate-lox2272

    Plasmid

    #162076

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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