Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

178,236 results

Showing: 9541 - 9560 of 178236 results

  1. pET30a-His6-LOXCAT

    Plasmid
    #140084
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.
    Depositor
    Vamsi Mootha
    Publication
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140084-plasmid-map-sequence-id-311164

    Enlarge

  2. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Publication
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Tags
    His6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159390-plasmid-map-sequence-id-310625

    Enlarge

  3. pLIX403-ccdB-Blast

    Plasmid
    #158560
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158560-plasmid-map-sequence-id-309876

    Enlarge

  4. pPP444

    Plasmid
    #158802
    Purpose
    Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.
    Depositor
    Jennifer Doudna
    Publication
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasPhi-3
    Expression
    Mammalian
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158802-plasmid-map-sequence-id-309578

    Enlarge

  5. pcDNA3.1.Gαi1-LgB91

    Plasmid
    #134342
    Purpose
    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαi1-LgB91 (GNAI1 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134342-plasmid-map-sequence-id-306400

    Enlarge

  11. pCMV-Sport6-CD81-pHluorin

    Plasmid
    #130903
    Purpose
    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD81-pHluorin (CD81 Human, aequorea victoria)
    Tags
    pHluorin
    Expression
    Mammalian
    Mutation
    pHluorin inserted between Leu-49 and Gly-50 in ex…
    Promoter
    CMV
    Available since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130903-plasmid-map-sequence-id-259050

    Enlarge

  19. pLentiGuide

    Plasmid
    #117986
    Purpose
    pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
    Depositor
    Paul Khavari
    Publication
    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31
    Insert
    sgRNA backbone (F+E)
    Use
    Lentiviral
    Available since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117986-plasmid-map-sequence-id-235371

    Enlarge

Showing: 9541 - 9560 of 178236 results