166,276 results

1. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-3xFLAG-TurboID-MCS

   Plasmid

   #186813

   Purpose

   CAG promoter-driven expression of TurboID-fused protein (N terminus)

   Depositor

   Masahito Ikawa

   Article

   Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

   Type

   Empty backbone

   Tags

   3xFLAG-TurboID

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Merlin

   Plasmid

   #84293

   Purpose

   Expression of wt Merlin

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Merlin (NF2) (NF2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIDTSmart-MbPylRS

   Plasmid

   #217361

   Purpose

   Expresses Methanosarcina barkeri aminoacyl-tRNA synthetases; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. barkeri pyrrolysyl-tRNA synthetase
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pORANGE LOX Cloning template vector

   Plasmid

   #139651

   Purpose

   Cloning template for ORANGE-CAKE conditional knock-in constructs

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCURE

   Plasmid

   #23013

   Depositor

   James Bowie

   Article

   Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

   Insert

   I-CreI homing endonuclease
   

   Expression

   Bacterial

   Mutation

   I-CreI expression is controlled by the arabinose …

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pOpen-SapIR

   Plasmid

   #165537

   Purpose

   Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   SapI
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE

   Plasmid

   #183522

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   rsChRmine-oScarlet
   

   Use

   AAV

   Mutation

   I146M/G174S

   Promoter

   CaMKIIa

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-G1TFAKRS

   Plasmid

   #177311

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHLmMBP-11

    Plasmid

    #72349

    Purpose

    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

    Depositor

    Luca Jovine

    Article

    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

    Type

    Empty backbone

    Tags

    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

    Expression

    Mammalian

    Promoter

    CMV enhancer + chicken beta-actin promoter

    Available Since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBoBi-hLAMP2-C-GC6s

    Plasmid

    #154151

    Purpose

    To detect the calcium ion releases close to the lysosomal surface

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

    Insert

    LAMP2 and GCamp6s (LAMP2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP566

    Plasmid

    #222724

    Purpose

    pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3-EF1aH N90-beta-catenin

    Plasmid

    #86499

    Purpose

    Mammalian expression of Myc tagged N90 beta-catenin

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90; constitutively acti…

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid

    #218205

    Purpose

    Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

    Depositor

    Katarzyna Koltowska

    Article

    Panara et al Development. 2024 May 1;151(9):dev202525

    Insert

    -2.1prox1a
    

    Use

    Zebrafish expression

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDESTmycQKI

    Plasmid

    #19870

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    quaking homolog, KH domain RNA binding (QKI Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pMEL13

    Plasmid

    #107919

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.3_KLF4

    Plasmid

    #26815

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

    Insert

    5'UTR-KLF4-3'UTR (KLF4 Human)
    

    Expression

    Mammalian

    Mutation

    Starts from second ATG

    Available Since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3/UBC9

    Plasmid

    #20082

    Depositor

    Edward Yeh

    Article

    Gong et al J Biol Chem. 1997 Nov 7;272(45):28198-20

    Insert

    UBC9 (UBE2I Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNP1954

    Plasmid

    #222730

    Purpose

    pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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