159,557 results

1. pBEVY-U

   Plasmid

   #51230

   Purpose

   bi-directional expression vector for yeast, constitutively active, URA3 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD/ADH1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PER2:Luc Plasmid

   Plasmid

   #206875

   Purpose

   Per2 promoter luciferase reporter

   Depositor

   Michelle Farkas

   Article

   Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

   Insert

   Period 2 (Per2 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-HA-USP49

   Plasmid

   #22586

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP49 (USP49 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pVAX1-BMP2

   Plasmid

   #137909

   Purpose

   Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

   Depositor

   Georg Feichtinger

   Article

   Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

   Insert

   BMP2 (BMP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR EGFR-CD3ε-FusionRed

   Plasmid

   #188626

   Purpose

   Human EGFR labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   EGFR (EGFR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   CD3ε-FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBD-SpyTag-noro-VLP

   Plasmid

   #165989

   Purpose

   Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

   Insert

   SpyTag-noro-VLP (ORF2 norovirus, Synthetic)
   

   Use

   Tags

   SpyTag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CaVα2δ1

   Plasmid

   #26575

   Depositor

   Diane Lipscombe

   Article

   Lin et al J Neurophysiol. 2004 Nov . 92(5):2820-30

   Insert

   Cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_MFE1_hrGFP

   Plasmid

   #84617

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. cfSGFP2-N

    Plasmid

    #37533

    Depositor

    Ikuo Wada

    Article

    Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

    Insert

    cysteine-free SGFP2-N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C48S and C70M mutations relative to SGFP2

    Promoter

    CMV

    Available since

    Aug. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKL2315

    Plasmid

    #187876

    Purpose

    Expresses Cre recombinase.

    Depositor

    Kan Wang

    Article

    Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-80L MBP-SspB Nano

    Plasmid

    #60409

    Purpose

    E. coli expression of SspB Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    SspB nano
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Y11K and A15E

    Promoter

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF463

    Plasmid

    #167671

    Purpose

    Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSET FLIPPi-200u

    Plasmid

    #13555

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi G162A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    G162A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK-rABE-Flag

    Plasmid

    #191383

    Purpose

    Doxycycline inducible expression of RNA A-to-I editor rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    rABE-Flag
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    tet-inducible promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLGR002 LGR Cas9 guide vector

    Plasmid

    #188320

    Purpose

    Universal backbone for CRISPR/Cas9 sgRNA expression

    Depositor

    Laralynne Przybyla

    Article

    Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    PuroR-T2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    pU6 5'CMV and EF1-alpha

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRAM18dRGA[MCS]

    Plasmid

    #84683

    Purpose

    Can be used for the transformation of rickettsia

    Depositor

    Ulrike Munderloh

    Article

    Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUSE2ss-CLIg-mK_M18

    Plasmid

    #82358

    Purpose

    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30b_OmpAT7Sav

    Plasmid

    #138589

    Purpose

    T7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Nature. 2016 Sep 29;537(7622):661-665. d

    Insert

    T7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
    

    Use

    Synthetic Biology

    Tags

    OmpA export signal and T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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