164,577 results

1. pAAV-SMVP-Cas9N

   Plasmid

   #80930

   Purpose

   Expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAJM.336

   Plasmid

   #108528

   Purpose

   LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

   Depositor

   Christopher Voigt

   Article

   Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

   Insert

   LacIAM + PTac-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   LacI Q18M A47V F161Y

   Promoter

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCLXEBR-pTF-cKit

   Plasmid

   #114293

   Purpose

   TET-inducible expression of c-Kit

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   cKit (Kit Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. tdTomato-pBAD

   Plasmid

   #54856

   Purpose

   Localization: Protein Expression Vector , Excitation: 554, Emission: 581

   Depositor

   Nathan Shaner, Michael Davidson, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   6xHis and tdTomato

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMslc

   Plasmid

   #32095

   Depositor

   Christopher Buck

   Article

   Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

   Insert

   SLC35A1 (SLC35A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   FerH

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSRK2706

   Plasmid

   #78571

   Purpose

   Expresses untagged human rhinovirus 3C protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

   Insert

   human rhinovirus 3C protease (HRVBgp1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2.2

   Plasmid

   #34885

   Depositor

   Irvin Chen

   Article

   Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

   Insert

   Sindbis virus envelope protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   SGN UTR in E1 domain

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2474

   Plasmid

   #144029

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ONECUT3 (ONECUT3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG-GFP/cre

    Plasmid

    #49054

    Purpose

    Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

    Depositor

    Fred Gage

    Article

    Tashiro et al Nat Protoc. 2006;1(6):3049-55.

    Insert

    GFP/Cre
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TRE-DIO-hM4Di-mCherry

    Plasmid

    #182532

    Purpose

    This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

    Depositor

    William Wisden

    Article

    Yu et al Science. 2022 Jul;377(6601):63-72. doi:

    Insert

    AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    hM4Di-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    tetO promoter

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPROBE-gfp[tagless]

    Plasmid

    #40164

    Depositor

    Steven Lindow

    Article

    Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    Promotor probe
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx4-H6-FGAm (FlincG3)

    Plasmid

    #49202

    Purpose

    Fluorescent reporter for cGMP

    Depositor

    John Garthwaite

    Article

    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do

    Insert

    FlincG3
    

    Use

    Tags

    His tag + other tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Met to Lys in cpEGFP region similar to CGaMP3

    Promoter

    CMV

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVhyg-3xT7-CA-ChREBP

    Plasmid

    #171029

    Purpose

    Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

    Depositor

    Kai Ge

    Article

    Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

    Insert

    CA-ChREBP (Mlxipl Mouse)
    

    Use

    Retroviral

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    amino acids 240 - 864

    Promoter

    5' LTR

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXW117Ptet2-gfp

    Plasmid

    #160818

    Purpose

    aTc-responsive single-layered circuit

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23117-30tetR-t-Ptet2-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23117, Ptet2

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBMN-HA-TBK1

    Plasmid

    #199205

    Purpose

    Used for the expression of TBK1 (SMC Internal No. 1996)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Klb-EGFP

    Plasmid

    #45531

    Depositor

    Zipora Yablonka-Reuveni

    Article

    Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

    Insert

    beta Klotho (Klb Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-Hygro-TRE>{MCS}:T2A:EGFP

    Plasmid

    #236250

    Purpose

    Hygromycin selected lentiviral vector with MCS for tetracycline-inducible gene, for use with dual-inducible CymR-CuO system

    Depositor

    Genevieve Kendall

    Article

    Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHTNW

    Plasmid

    #136403

    Purpose

    Expression of N-terminal HaloTag-fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9-Tet1-CD

    Plasmid

    #83340

    Purpose

    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

    Depositor

    Ronggui Hu

    Article

    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

    Insert

    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    U6, CBH

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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