162,109 results

1. pcDNA3-HA-RIPK1

   Plasmid

   #78834

   Purpose

   To overexpress RIPK1 in Mammalian Cell

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-C1-mTrim21

   Plasmid

   #105516

   Purpose

   Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-TurboID-NES_pLX304

   Plasmid

   #202008

   Purpose

   expresses TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ClopHensor

   Plasmid

   #25938

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   ClopHensor
   

   Use

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Promoter

   Available since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. fabp10a:CreERT2; cryaa:mCerulean

   Plasmid

   #230044

   Purpose

   Inducible Cre expression in Zebrafish hepatocytes

   Depositor

   Sumeet Pal Singh

   Article

   Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

   Insert

   CreERT2
   

   Use

   Cre/Lox; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   fabp10a

   Available since

   Feb. 11, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOpen-phi29pol

   Plasmid

   #165573

   Purpose

   DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   phi29 DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJG100

   Plasmid

   #138966

   Purpose

   Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   wrmScarlet1-10
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-FOXO3a WT

   Plasmid

   #8360

   Depositor

   Michael Greenberg

   Article

   Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

   Insert

   FOXO3a (FOXO3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGM47523

   Plasmid

   #153221

   Purpose

   Level 0 zCas9i vector

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Insert

   SpCas9 with introns and 2 NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FEMP

    Plasmid

    #191973

    Purpose

    FRET-based indicator of ER-mitochondria proximity (FEMP)

    Depositor

    Luca Scorrano

    Article

    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    AkapYFP-Tav2a-CFP-sac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pttq18

    Plasmid

    #69122

    Purpose

    regulated expression of genes in Escherichia coli

    Depositor

    Michael Stark

    Article

    Stark et al Gene. 1987;51(2-3):255-67.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEF-GalNAc-T2-msGFP2

    Plasmid

    #140164

    Purpose

    Expresses the Golgi marker GalnacT2-msGFP2

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    GalNAc-T2-msGFP2 (GALNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBpaRS3tRNA

    Plasmid

    #197102

    Purpose

    To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

    Insert

    TyrRS variant, amber suppressor tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1 EGFP-3XNLS

    Plasmid

    #58468

    Purpose

    Expresses a nuclear-targeted eGFP on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Insert

    3X NLS with 3X stop codons
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.hSyn.iGluSnFR3.v857.GPI

    Plasmid

    #178331

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-25xERSE2-minP-EGFP

    Plasmid

    #159667

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xERSE2-minP-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid

    #174723

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    pac
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and T2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB1_alpha1

    Plasmid

    #68224

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD2sE_SC12

    Plasmid

    #219719

    Purpose

    DV2 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV2 sE SC12
    

    Use

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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