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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,557 results

Showing: 9641 - 9660 of 159557 results

  1. pAAV.CMV.ffLuciferase.SV40

    Plasmid
    #105532
    Purpose
    AAV expression of firefly luciferase from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105532-plasmid-map-sequence-id-202366

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  3. pET22b_PstS_3

    Plasmid
    #78200
    Purpose
    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A17C,I76G,A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A17C,I76G,A197C (please see depositor comment bel…
    Promoter
    T7
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    78200-plasmid-map-sequence-id-146050

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  6. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Promoter
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

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  7. pDONR221-SLC25A12_STOP

    Plasmid
    #161104
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC25A12 (SLC25A12 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161104-plasmid-map-sequence-id-421010

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  8. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid
    #225756
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Article
    Walton et al bioRxiv 2024.03.17.585235
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225756-plasmid-map-sequence-id-446064

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  11. pINDUCER20_mGFP

    Plasmid
    #190027
    Purpose
    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mEGFP
    Depositor
    Andrew Tutt
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    190027-plasmid-map-sequence-id-377385

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  12. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  13. pFLAG-CMV2-Hsp27-S15D/S78D/S82D

    Plasmid
    #85188
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S15D/S78D/S82D, phosphomimicking mutation
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85188-plasmid-map-sequence-id-167884

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Showing: 9641 - 9660 of 159557 results