Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

piggyBac CMV-GFP-FRT
(Plasmid #92078)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92078 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCyL50
  • Backbone manufacturer
    Sanger institute
  • Backbone size w/o insert (bp) 3050
  • Vector type
    Mammalian Expression ; piggyBac
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV-eGFP and TK-Neo between FRT3 and FRT
  • Insert Size (bp)
    4000
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T3, M13R
  • 3′ sequencing primer M13F(-20)
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Sanger institute (http://www.sanger.ac.uk/ technology/clonerequests)
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggyBac CMV-GFP-FRT was a gift from Jae Yong Han (Addgene plasmid # 92078 ; http://n2t.net/addgene:92078 ; RRID:Addgene_92078)
  • For your References section:

    Site-specific recombination in the chicken genome using Flipase recombinase-mediated cassette exchange. Lee HJ, Lee HC, Kim YM, Hwang YS, Park YH, Park TS, Han JY. FASEB J. 2016 Feb;30(2):555-63. doi: 10.1096/fj.15-274712. Epub 2015 Oct 6. 10.1096/fj.15-274712 PubMed 26443821