164,577 results

1. pLJC6-2XFLAG-TMEM192

   Plasmid

   #104435

   Purpose

   Expresses 2XFLAG-TMEM192 in mammalian cells

   Depositor

   David Sabatini

   Article

   Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

   Insert

   TMEM-192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   2XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF-G1(6/7FTrp)RS

   Plasmid

   #207621

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIB-HIS-TEV-CKS1

   Plasmid

   #177013

   Purpose

   Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

   Depositor

   Andrea Musacchio

   Article

   Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

   Insert

   CKS1 (CKS1B Human, Synthetic)
   

   Use

   Tags

   Polyhistidine-TEV

   Expression

   Insect

   Mutation

   codon-optimised for insect cell expression

   Promoter

   Available Since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJC49

   Plasmid

   #133060

   Purpose

   Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CL20_mEGFP_EWSR1_ERG

   Plasmid

   #205819

   Purpose

   Express mEGFP-tagged fusion protein, EWSR1_ERG from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   EWSR1_ERG (EWSR1, ERG Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3/Flag-METTL14

   Plasmid

   #53740

   Purpose

   For expression of METTL14 (methyltransferase-like 14) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 14 (METTL14 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. cytoplasmic cpVenus control

   Plasmid

   #186788

   Purpose

   Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic cpVenus control
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. mVenus C1

   Plasmid

   #27794

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus C1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-VAPB

   Plasmid

   #108126

   Purpose

   ER marker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:VAPB (VAPB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-hSyn-ASAP4e-Kv-WPRE

    Plasmid

    #201031

    Purpose

    Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

    Depositor

    Michael Lin

    Article

    Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

    Insert

    ASAP4e-Kv
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_816

    Plasmid

    #201157

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D908A

    Promoter

    EFS

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Gα13*-BERKY3

    Plasmid

    #158218

    Purpose

    BRET biosensor for detection of Gα13-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-PRG(RH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLV 4xSTAR-sTomato-NLS-blast

    Plasmid

    #136259

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGint

    Plasmid

    #24217

    Depositor

    Mariano Garcia-Blanco

    Article

    Bonano et al Nat Protoc. 2007 . 2(9):2166-81.

    Insert

    pGint
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Modified adenovirus in GFP

    Promoter

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-mNINJ1-GFP

    Plasmid

    #208775

    Purpose

    Inducibly expresses mouse NINJ1 tagged with GFP

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    mNINJ1 (Ninj1 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE_hygro_mRFP1_NRF2

    Plasmid

    #136579

    Purpose

    mammalian expression of mRFP1-tagged human NRF2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid

    #175184

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge