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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9701 - 9720 of 167600 results

  1. pTF101.1gw1

    Plasmid
    #134768
    Purpose
    A binary plasmid for gateway 1-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialaphos resistance gene (bar) for plant transformation
    Depositor
    Kan Wang
    Article
    et al Euphytica (2004) 136: 167.
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    134768-plasmid-map-sequence-id-266108

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  2. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  4. Cavb3

    Plasmid
    #26574
    Depositor
    Diane Lipscombe
    Insert
    calcium channel beta subunit-III (Cacnb3 Rat)
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only
    26574-plasmid-map-sequence-id-148124

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  5. pmVen-RecQL4

    Plasmid
    #211401
    Purpose
    mammalian expression of human RecQL4 N-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    RecQL4 (RECQL4 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211401-plasmid-map-sequence-id-420509

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  8. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  11. pSLIK-Neo

    Plasmid
    #25735
    Purpose
    3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Neomycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Available Since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25735-plasmid-map-sequence-id-201234

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  12. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    58310-plasmid-map-sequence-id-89822

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Showing: 9701 - 9720 of 167600 results