166,276 results
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pcDNA3-MEK5DD-HA
Plasmid#65247PurposeExpression of HA tagged dominant active MEK5 in mammalian cellsDepositorInsertMEK5DD (MAP2K5 Human)
TagsHAExpressionMammalianMutationchanged Ser311 to Asp, Ser315 to AspPromoterCMVAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-mRuby3
Plasmid#74252PurposeMammalian expression of tandem fusion of mClover3-mRuby3DepositorInsertmClover3-mRuby3
ExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD8-GFP-FKBP
Plasmid#100751PurposeExpression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.DepositorAvailable SinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
GW1-rogRFP2
Plasmid#207876PurposeMammalian expression of the red fluorescent rogRFP2 redox sensorDepositorInsertrogRFP2
ExpressionMammalianPromoterCMVAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
E228Q Vps4A-HA
Plasmid#200087Purpose3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vectorDepositorInsertVPS4A (VPS4A Human)
UseLentiviralTags3xHAExpressionMammalianMutationE228QPromoterCMVAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2 mBAX
Plasmid#129577PurposeCRISPR deletion of murine BAXDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-EGFP-T2A-Bsd
Plasmid#193198PurposeLentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance markerDepositorInsertEGFP
UseLentiviralExpressionMammalianPromoterEFSAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
Plasmid#129631PurposeVisualization of Akt and ERK activityDepositorInsertH2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
ExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRD215
Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-myc T58A
Plasmid#19763DepositorAvailable SinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-MQ1(Q147L)-EGFP
Plasmid#89793PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPR and LentiviralTags3*FLAG and T2A-EGFPExpressionMammalianMutationQ147L, CAA-->CTAPromoterhUbCAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRN11
Plasmid#84455Purposeshuttle plasmid for sarA P1-mCherry expressionDepositorInsertmCherry
UseShuttle vector e.coli-s.aureus, expression of sgf…Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDM1081
Plasmid#108981PurposeKnockout cassette HygromycinDepositorInsertKnockout cassette Hygromycin
UseCre/Lox; Dictyostelium discoideum knockout vectorPromotercoaAAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xFLAG-HSP27
Plasmid#129229PurposeEncodes FLAG tagged human HSP27 for purification by GST affinityDepositorAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EGFP-IRES(Neo)R
Plasmid#158068PurposeeGFP under the TRE3G promoter, Neo resistance. For cloning GOIDepositorInserteGFP
TagseGFPExpressionMammalianPromoterTRE3GAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab1aWT
Plasmid#46776Purposeexpression of WT Rab1a in mammalian cellsDepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2-GFP
Plasmid#202194Purposeknock-in donor vector of GFP to the C-terminus of endogenous human PLIN2DepositorAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only
