164,577 results

1. pcDNA3/Flag-METTL14

   Plasmid

   #53740

   Purpose

   For expression of METTL14 (methyltransferase-like 14) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 14 (METTL14 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTK305

   Plasmid

   #66812

   Purpose

   IVT vector for Fluc mRNA

   Depositor

   Ron Weiss

   Article

   Andries et al J Control Release. 2015 Sep 2. pii: S016

   Insert

   Firefly luciferase
   

   Use

   Luciferase; Mrna ivt

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mVenus C1

   Plasmid

   #27794

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus C1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_BiSSTe4_GCaMP6f

   Plasmid

   #213947

   Purpose

   AAV construct expressing GCaMP6f driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_GCaMP6f

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Has Service

   AAV PHP.eB and AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCC001

   Plasmid

   #124451

   Purpose

   Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

   Depositor

   John Morrissey

   Article

   Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

   Insert

   HH-BsaI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   gRNA expression cassette from pUDP002 modified to…

   Promoter

   TDH3 promoter from S.cerevisiae (already present …

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN FKBP(DD)-YFP

   Plasmid

   #31763

   Depositor

   Thomas Wandless

   Article

   Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

   Insert

   FKBP(DD)-YFP-HA (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   F36V, L106P on FKBP12

   Promoter

   LTR

   Available Since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. L0-I35 p35S-XVE-pEstradiol GG0-GG1

   Plasmid

   #195904

   Purpose

   Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   p35S-XVE-LexA-p35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE PGK-GFP-IRES-LUC-W

   Plasmid

   #46793

   Purpose

   2nd generation lentiviral vector

   Depositor

   Darrell Kotton

   Article

   Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

   Inserts

   PGK
   eGFP (eGFP )
   IRES
   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. p31sheLL

    Plasmid

    #37322

    Depositor

    John Schiller

    Article

    Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

    Insert

    HPV31L1+L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid

    #61358

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-Ataxin3Q84

    Plasmid

    #22123

    Depositor

    Henry Paulson

    Article

    Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-VAPB

    Plasmid

    #108126

    Purpose

    ER marker

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:VAPB (VAPB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSRK2706

    Plasmid

    #78571

    Purpose

    Expresses untagged human rhinovirus 3C protease in E. coli

    Depositor

    David Waugh

    Article

    Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

    Insert

    human rhinovirus 3C protease (HRVBgp1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHO4d-Cas9

    Plasmid

    #67881

    Purpose

    Cas9nlsHis6 Bacterial Expression construct

    Depositor

    Michael Nonet

    Article

    Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and T7 epitope

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYFP-RPB1aAmr

    Plasmid

    #75284

    Purpose

    Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

    Depositor

    Xavier Darzacq, Robert Singer

    Article

    Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

    Insert

    POLR2A (POLR2A Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    N792D alpha-amanitin–resistant

    Promoter

    CMV

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJRH-1364 LV EF1a-CD4 IRES-EGFP

    Plasmid

    #201920

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD4

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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