166,276 results

1. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

   Plasmid

   #170273

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
   

   Expression

   Mammalian

   Mutation

   See Comments

   Promoter

   CMVd1

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-CD81-10

   Plasmid

   #54031

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   He et al PLoS Pathog. 2013;9(10):e1003701. doi: 1

   Insert

   CD81 (CD81 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRDA_478

   Plasmid

   #179096

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (SpG)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HypoxCR

   Plasmid

   #59946

   Purpose

   A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

   Depositor

   Anne Le

   Article

   Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

   Inserts

   GFP-Pest
   mCherry-Geminin
   

   Use

   Lentiviral

   Tags

   FLAG and PEST

   Expression

   Mammalian

   Promoter

   CMV and min CMV

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-T5gene122

   Plasmid

   #165544

   Purpose

   Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   T5 DNA Polymerase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 9, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. VRQR-ABEmax

   Plasmid

   #119811

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCIneoGFP-BMI1

   Plasmid

   #128328

   Purpose

   Mammalian expression vector of GFP-tagged human BMI1

   Depositor

   Yutaka Hata

   Article

   Hossain et al Mol Cell Biol. 2018 Aug 15;38(17). pii:

   Insert

   BMI1 (BMI1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-APOBEC1-YTH

   Plasmid

   #131636

   Purpose

   Expresses APOBEC1 fused to the YTH domain

   Depositor

   Kate Meyer

   Article

   Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

   Insert

   APOBEC1 fused to YTH domain (YTHDF2 Rat, Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-rxRFP

   Plasmid

   #67830

   Purpose

   Redox-Sensitive Red Fluorescent Protein

   Depositor

   Huiwang Ai

   Article

   Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

   Insert

   Redox-Sensitive Red Fluorescent Protein
   

   Tags

   His Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG006 (Ec67 retron)

    Plasmid

    #157884

    Purpose

    Expresses the Ec67 retron defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Ec67 retron
    

    Expression

    Bacterial

    Available Since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX302-MKP5-V5 puro

    Plasmid

    #87770

    Purpose

    Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GluA1-scFV-Halo

    Plasmid

    #230065

    Purpose

    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag. Can be used to produce scFv in HEK cells or directly transfected into neurons.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-SfiIR (cDNA)

    Plasmid

    #165528

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    SfiI (used to create cDNA libraries)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. ER-SPLICSs-cFAST10

    Plasmid

    #210431

    Purpose

    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    ER-SPLICSs-cFAST10
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HDM-tat1b

    Plasmid

    #204154

    Purpose

    Lentivirus helper plasmid coding for Tat

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    tat
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-myc-Miro2

    Plasmid

    #47891

    Purpose

    Expresses myc tagged Miro2

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro2 (RHOT2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIN4-EF2-ABCG2-IRES-Neo

    Plasmid

    #25983

    Depositor

    Ren-he Xu

    Article

    Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

    Insert

    ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)
    

    Use

    Lentiviral

    Tags

    EF1-apha promoter and IRES-Neo

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLI50

    Plasmid

    #13573

    Depositor

    Chia Lee

    Article

    Lee et al Gene. 1991 Jul 15. 103(1):101-5.

    Type

    Empty backbone

    Use

    S. aureus-e. coli shuttle vector

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJA54 HLA-A*03:01

    Plasmid

    #180451

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*03:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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