166,276 results

1. TFORF1072

   Plasmid

   #141790

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFATC4 (NFATC4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. V72 ELP

   Plasmid

   #68938

   Purpose

   Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

   Depositor

   Andrew MacKay

   Article

   Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

   Insert

   V72 ELP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pF(UG) hSyn SYP-mRuby3

   Plasmid

   #179727

   Purpose

   Lentiviral expression of SYP-mRuby3 for use as a synaptic vesicle marker

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   SYP (Syp Mouse)
   

   Use

   Lentiviral

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

   Plasmid

   #100157

   Purpose

   Expresses MUS81

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   MUS81 (MUS81 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV_RBMX-eA3A-UdgX-HF-nCas9

   Plasmid

   #163548

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-HF-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJV53

   Plasmid

   #26904

   Depositor

   Graham Hatfull

   Article

   van Kessel et al Nat Methods. 2007 Feb . 4(2):147-52.

   Insert

   Che9c genes 60-61
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Nrf1-Myc pcDNA3.1(+)-C-Myc

   Plasmid

   #169125

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pInt_attP1_LCS_kanR

   Plasmid

   #205300

   Purpose

   Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO.1 - TRC mTagBFP2

   Plasmid

   #191566

   Purpose

   Expresses an shRNA and mTagBFP2

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T-E2-25K

    Plasmid

    #63572

    Purpose

    Expression of human Ubiquitin conjugating enzyme E2-25K

    Depositor

    Titia Sixma

    Article

    Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

    Insert

    UBE2-25K (UBA7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Isoform 1 (200 resiudes)

    Promoter

    tac

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. mCh-Rab7A

    Plasmid

    #61804

    Purpose

    Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145

    Plasmid

    #111731

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q145 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHIV-ARF-zsGreen

    Plasmid

    #110729

    Purpose

    Mammalian Expression of p14ARF IRES zsGreen

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    p14ARF IRES zsGreen (CDKN2A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 iCasper T2A HO1

    Plasmid

    #64278

    Purpose

    Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-TEK-V5/HIS

    Plasmid

    #205611

    Purpose

    Expression of human TEK receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TEK receptor tyrosine kinase (TEK Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHIV-iRFP720-E2A-Luc

    Plasmid

    #104587

    Purpose

    Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

    Depositor

    Antonius Plagge

    Article

    Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

    Insert

    iRFP720-E2A-Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    bi-cistronic

    Expression

    Mammalian

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RUSH reporter-HMGB1-SBP-GFP

    Plasmid

    #172357

    Purpose

    Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

    Depositor

    Guido Kroemer

    Article

    Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

    Insert

    high mobility group box 1 (HMGB1 Human)
    

    Use

    Lentiviral

    Tags

    SBP-GFP

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS

    Plasmid

    #12158

    Depositor

    Inder Verma

    Article

    Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. His-TEV-SNAP-PRC1

    Plasmid

    #167541

    Purpose

    For expression of His6-TEV-SNAP-PRC1 in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

    Insert

    PRC1 (PRC1 Human)
    

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge