175,192 results

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MAC-NRP1

Plasmid

#158384

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

NRP1 (NRP1 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX307-FURIN-puro

Plasmid

#158460

Purpose

Constitutive lentiviral expression of human FURIN.

Depositor

Alejandro Chavez, Sho Iketani

Insert

FURIN (FURIN Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET30a_HIV-RT

Plasmid

#153311

Purpose

Bacterial expression of HIV reverse transcriptase

Depositor

Bryan Dickinson, Drew Endy

Insert

HIV Reverse Transcriptase

Tags

His

Expression

Bacterial

Available Since

July 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-mRuby2-Gephyrin.FingR-IL2RGTC

Plasmid

#125696

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mRuby2-Gephyrin.FingR-IL2RGTC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBA950

Plasmid

#122239

Purpose

CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.

Depositor

Jonathan Weissman

Article

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Mfn2-HA

Plasmid

#139192

Purpose

Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

Depositor

Allan Weissman

Article

Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

Insert

MFN2 (MFN2 Human)

Tags

3HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-M2

Plasmid

#135583

Purpose

High eukaryotic protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-mDNMT1_nick-lenti

Plasmid

#135959

Purpose

S. pyogenes nicking sgRNA for mouse DNMT1

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

mDNMT1_nick

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-NPC1

Plasmid

#132295

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

NPC1 (NPC1 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-ymNeongreen-CaURA3

Plasmid

#125703

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059 pFA6a-link-ymNeongreen-URA3.

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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HPV16 E6 TCR

Plasmid

#122727

Purpose

Express HPV16 E6 TCR

Depositor

Christian Hinrichs

Article

Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

Insert

HPV16 E6 TCR

Use

Retroviral

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CTCF

Plasmid

#116728

Purpose

Lentiviral expression of CTCF

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CTCF (CTCF Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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BPK2014-AaCas12b

Plasmid

#121949

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

10xHis tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_FOXA2_P2A_Hygro_Barcode

Plasmid

#120439

Purpose

Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

FOXA2 (FOXA2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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3xHA-miniTurbo_pAS32

Plasmid

#118222

Purpose

expresses 3xHA-tagged miniTurbo in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Tags

3x HA tag

Expression

Worm

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

ges-1p

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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TNFR1-YFP

Plasmid

#111209

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

TNFRSF1A (TNFRSF1A Human)

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pV1524

Plasmid

#111431

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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