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eGFP-KIF5B

Plasmid

#172203

Purpose

Expresses KIF5B tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Cavb3

Plasmid

#26574

Depositor

Diane Lipscombe

Insert

calcium channel beta subunit-III (Cacnb3 Rat)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP

Plasmid

#50716

Purpose

5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

split EGFP

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pSNAPf-ADRβ2 Control Plasmid

Plasmid

#101123

Purpose

Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Insert

Beta-2-Adrenergic Receptor (ADRB2 Human)

Tags

SNAP-tag (SNAPf)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

Plasmid

#207953

Purpose

nuclease inactive control for TRF1FokI WT

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI (D450A)

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSL21-mCherry

Plasmid

#164410

Purpose

Retroviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Article

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-SpCas9-NG

Plasmid

#117919

Purpose

Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mCherry from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

Plasmid

#140220

Purpose

Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

dLbCpf1

Use

CRISPR

Tags

miniVPR-mCherry

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-CreN-InteinN

Plasmid

#187614

Purpose

AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Article

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

CreN-InteinN

Use

AAV

Expression

Mammalian

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CRBN-P2A-Hygro

Plasmid

#124303

Purpose

Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

CRBN (CRBN Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-3C-sGFP11_pcDNA3.1(-)

Plasmid

#182932

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 11, FLAG tag, and HA signa…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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CRY2olig-TDP-43

Plasmid

#218920

Purpose

Expression of CRY2olig-mCherry fused to TDP-43

Depositor

Tatyana Shelkovnikova

Insert

CRY2olig-mCherry tagged TDP-43

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPAGFP-N1

Plasmid

#11909

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

pPAGFP-N1

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCART0016_CROPseq-CAR-Puro_GPC3-BBz

Plasmid

#242985

Purpose

GPC3-BBz CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GPC3-BBz CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0035_CROPseq-CAR-Puro_CD19-28z

Plasmid

#242983

Purpose

CD19-28z FMC63 CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-28z FMC63 CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR V2 sgCGAS_1

Plasmid

#241454

Purpose

Delete CGAS

Depositor

Richard Possemato

Insert

sgCGAS_1 (CGAS Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE8

Plasmid

#90342

Purpose

FOXO1 - Insulin (PI3K/AKT) gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

FOXO

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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