165,256 results

1. pMDC32-HPB

   Plasmid

   #32078

   Depositor

   Fumi Katagiri

   Article

   Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

   Type

   Empty backbone

   Use

   Gateway destination binary vector

   Tags

   HA-Prescission-Biotin

   Promoter

   35S promoter

   Available Since

   Dec. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDule-Tet2.0

   Plasmid

   #85496

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScarlet-N1

   Plasmid

   #128060

   Purpose

   Mammalian expression vector for C terminal mScarlet-I fusions

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Tags

   mScarlet-I

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218862

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZR069_CROPseq-sgPTPRC-CRISPRi

   Plasmid

   #180271

   Purpose

   Lentiviral CROPseq vector expressing PTPRC (CD45) targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   PTPRC (CD45) sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21 MBPx-SpyCatcher

   Plasmid

   #72327

   Purpose

   Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET21 MBPx-SpyCatcher
   

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   MBP contains A312V, I317V mutations and 172, 173,…

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGAselect

   Plasmid

   #195714

   Purpose

   pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Feb. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. HA-mDyn3 pcDNA3

   Plasmid

   #36265

   Depositor

   Pietro De Camilli

   Article

   Ferguson et al Science. 2007 Apr 27;316(5824):570-4.

   Insert

   Dynamin 3 (Dnm3 Mouse)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 20, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCMVTNT-T7-KPNA7

   Plasmid

   #26683

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 7 (KPNA7 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. hE-cadherin/Δp120

    Plasmid

    #45770

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Δp120 (CDH1 Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 762-764 converted from EED to AAA to …

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

11. HLA_A2402

    Plasmid

    #165010

    Purpose

    Expression of HLA-A*24:02 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLHKI-pGFPN3

    Plasmid

    #21917

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKI in pGFP-N3 (HK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is full length human HKI in pGFP-N3

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF.myc.ER-E2-Crimson

    Plasmid

    #38770

    Purpose

    In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

    Depositor

    Benjamin Glick

    Article

    Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

    Insert

    E2-Crimson
    

    Tags

    ER retention signal (KDEL), ER signal sequence (M…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX458-Ef1a-Cas9-H2B-mCherry

    Plasmid

    #159655

    Purpose

    Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE-Medium-FT

    Plasmid

    #31914

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Medium-FT
    

    Expression

    Mammalian

    Mutation

    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid

    #113950

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 21-852

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-P07

    Plasmid

    #124788

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GfaABC1D-jGCaMP8m

    Plasmid

    #213010

    Purpose

    To study calcium signaling in Astrocytes

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    GfaABC1D-jGCaMP8m
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-eGFP-53BP1

    Plasmid

    #60813

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Dec. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAC1-V16-hGDNF

    Plasmid

    #122035

    Purpose

    AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

    Depositor

    Liliane Tenenbaum

    Article

    Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

    Insert

    hGDNF (GDNF Human)
    

    Use

    AAV

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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