162,682 results

1. psiCHECK-2-Fhod1-3'UTR

   Plasmid

   #61773

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Fhod1

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Fhod1 3'UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

2. psiCHECK-2-Mapk12-3'UTR

   Plasmid

   #61783

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Mapk12

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Mapk12 3'UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCEP4-hERG1a

   Plasmid

   #53052

   Purpose

   Expresses alpha subunit of hERG1a K channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

   Insert

   Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. JD689

   Plasmid

   #160396

   Purpose

   citrus GRF4-GIF1 in pDONR-zeo

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Insert

   citrus GRF4-GIF1 chimera
   

   Use

   Entry clon

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN7HELIX

   Plasmid

   #190662

   Purpose

   Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   CMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-PalmGRET

   Plasmid

   #158221

   Purpose

   BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.

   Depositor

   Charles P. Lai

   Article

   Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

   Insert

   PalmGFP-Nluc
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MultiMate-Cell-cycle

   Plasmid

   #206280

   Purpose

   Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
   

   Use

   Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH3

   Plasmid

   #84453

   Purpose

   shuttle plasmid for sarA P1-YFP expression

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   YFP venus
   

   Use

   Shuttle vector e.coli-s.aureus, expression of sgf…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW-H1 empty vector

   Plasmid

   #25870

   Purpose

   3rd generation lentiviral vector

   Depositor

   Sally Temple

   Article

   Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CAG-miniGFP1-P2A-FusionRed

    Plasmid

    #188732

    Purpose

    Express miniGFP1 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG1237 FIREmate-ECFP-Fis1

    Plasmid

    #205173

    Purpose

    Expresses FIREmate-ECFP-Fis1 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Fis1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGW_RUBY_EggCas9

    Plasmid

    #225990

    Purpose

    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-FLAG

    Plasmid

    #183511

    Purpose

    Gateway converted, destination vector with N-terminal Flag tag. For mammalian expression.

    Depositor

    Emanuele Panza

    Article

    Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. V5-BS2-NLS (Nucleoplasmin)

    Plasmid

    #220342

    Purpose

    Mammalian expression vector BS2 in nucleus

    Depositor

    Bryan Dickinson

    Article

    Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

    Insert

    BS2
    

    Use

    Tags

    NLS (Nucleus localization sequence) and V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. NSarQ27G-VMAn-FKBP

    Plasmid

    #70230

    Purpose

    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pmCherry_a_tubulin_IRES_puro2

    Plasmid

    #21043

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV MP-135 mRFP

    Plasmid

    #206927

    Purpose

    The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MP-135

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-HP1gamma

    Plasmid

    #17650

    Depositor

    Tom Misteli

    Article

    Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

    Insert

    HP1 gamma (CBX3 Human)
    

    Use

    Tags

    GFP and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIP-CMV-mCherry-53BP1

    Plasmid

    #127658

    Purpose

    Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge