179,813 results

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LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Publication

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#161927

Purpose

Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Publication

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Surface marker and Citrine repression reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pUC19_CRISPR_DpmrA

Plasmid

#160903

Purpose

CRISPR_Cas9, lambda red recombineering

Depositor

Anne-Catrin Uhlemann

Publication

McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9
Lambda Red Recombineering Genes
Zeocin Resistance Cassette

Promoter

araBAD

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Publication

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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lenti SYN-dCas9-KRAB-MeCP2

Plasmid

#155365

Purpose

Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

Depositor

Jeremy Day

Publication

Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

Insert

dCas9-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

3x FLAG and 7x His

Expression

Mammalian

Promoter

SYN

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-hACE2-hygro

Plasmid

#161758

Purpose

Expresses human ACE2 in mammalian cells

Depositor

Neville Sanjana

Publication

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Human ACE2 (ACE2 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Promoter

EF1a

Available since

Nov. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

7 Articles

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pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Publication

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Tags

Fc tag and His tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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SNAP-Sec61B

Plasmid

#141152

Purpose

Fluorescent labeling of ER membrane in mammalian cells

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec61 beta (SEC61B Human)

Tags

SNAP tag

Expression

Mammalian

Mutation

L79M on Sec61B

Promoter

CMV

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAC-NRP1

Plasmid

#158384

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NRP1 (NRP1 Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Publication

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-Puro-mGSDME

Plasmid

#154877

Purpose

Expresses mGSDME in mammalian cells

Depositor

Judy Lieberman

Publication

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-gRNA-Actb1216

Plasmid

#155368

Purpose

PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PspCas13b gRNA targeting Actb 1216

Use

CRISPR

Expression

Mammalian

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-Tet

Plasmid

#138086

Purpose

Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

Depositor

Eva Frickel

Publication

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-X1-Neo-HA-UFL1

Plasmid

#139860

Purpose

Lentiviral expression of HA-tagged human UFL1

Depositor

Jacob Corn

Publication

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1 (UFL1 Human)

Use

Lentiviral

Tags

HA

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Publication

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Publication

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

T7 Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pU6-sp-sgRNA-mDNMT1_nick-lenti

Plasmid

#135959

Purpose

S. pyogenes nicking sgRNA for mouse DNMT1

Depositor

David Liu

Publication

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mDNMT1_nick

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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