165,255 results

1. FAS promoter luciferase

   Plasmid

   #8890

   Depositor

   Bruce Spiegelman

   Article

   Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

   Insert

   Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-opto-mRipK3

   Plasmid

   #208783

   Purpose

   Inducibly expresses mouse RipK3 fused to light activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mRipK3 Cry2 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG mito-RCaMP1h

   Plasmid

   #105013

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   RCaMP1h
   

   Expression

   Mammalian

   Promoter

   pCAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

   Plasmid

   #234846

   Purpose

   3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

   Depositor

   Malin Parmar

   Article

   Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 Blast NT shRNA

   Plasmid

   #123306

   Purpose

   Expression of a non-targeting shRNA

   Depositor

   Giovanni Tonon

   Article

   Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

   Insert

   Non-targeting shRNA
   

   Use

   Lentiviral and RNAi

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. epi-BE4max-NG

   Plasmid

   #135977

   Purpose

   Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

   Depositor

   Yongming Wang

   Article

   Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

   Plasmid

   #185911

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8e-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hChATp-tdTomato

   Plasmid

   #197564

   Purpose

   Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

   Depositor

   Casey Maguire

   Article

   Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   human ChAT promoter

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-Myc-Parkin

    Plasmid

    #17612

    Depositor

    Ted Dawson

    Article

    Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

    Insert

    Parkin (PRKN Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEHIShqprt

    Plasmid

    #92166

    Purpose

    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    Quinolinate Phosphoribosyltransferase (QPRT Human)
    

    Tags

    TEV protease -cleavable N-terminal 6His

    Expression

    Bacterial

    Mutation

    changed Asp 2 to Asn to create BspHI site

    Promoter

    T7

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRC-APEX2

    Plasmid

    #72558

    Purpose

    Expression of cytosolic APEX2 in bacteria

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    A134P relative to APEX

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid

    #148120

    Purpose

    Mammalian Expression of HshnRNPH1

    Depositor

    Elisa Izaurralde

    Insert

    HshnRNPH1 (HNRNPH1 Human)
    

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Tags

    EGFP

    Expression

    Yeast

    Promoter

    pBAD/tetO

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn5-165

    Plasmid

    #118545

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR SFFVp YFP-PIF-SOS2cat

    Plasmid

    #50851

    Purpose

    Optogenetic Ras activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Inserts

    SOS2cat (SOS2 Human)
    PIF6 1-100
    

    Use

    Lentiviral

    Tags

    YFP

    Mutation

    residues 566–1049

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-sgRNA-RFP

    Plasmid

    #166005

    Purpose

    Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

    Depositor

    Martin Buck

    Article

    Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-H2BeGFP

    Plasmid

    #21210

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBad-EBFP2

    Plasmid

    #14891

    Purpose

    Bacterial expression of EBFP2 blue fluorescent protein

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 May 22;46(20):5904-10

    Has Service

    Cloning Grade DNA

    Insert

    EBFP2
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Available Since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNFkB:eGFP

    Plasmid

    #44922

    Depositor

    John Rawls

    Article

    Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

    Insert

    EGFP
    

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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