165,307 results

1. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #106895

   Purpose

   Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG Lenti CMV Empty Puro

   Plasmid

   #236083

   Purpose

   Empty lentiviral backbone

   Depositor

   Melina Fan

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   April 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHAGE-AXL

   Plasmid

   #116714

   Purpose

   Lentiviral expression of AXL

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Mutation

   WT

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

   Plasmid

   #176282

   Purpose

   Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   TeNT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin I

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

   Plasmid

   #207491

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Fusion protein with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP13897 - pAAV-AiE0671m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3897)

   Plasmid

   #224038

   Purpose

   AiE0671m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L4/5_IT neurons

   Depositor

   Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS1993

   Plasmid

   #239836

   Purpose

   OMEGAOff-KRK mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
   

   Tags

   HA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1 + E137K/E4…

   Promoter

   CMV

   Available Since

   July 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-AURKB

   Plasmid

   #23682

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   AURKB (AURKB Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. R777-E318 Hs.SOS1-nostop

    Plasmid

    #70602

    Purpose

    Gateway ORF clone of human SOS1 [NM_005633.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SOS1 (SOS1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. vGlut1 peptide- GST

    Plasmid

    #218677

    Purpose

    Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    vGlut (Slc17a6 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. KW57_HART_RAC1

    Plasmid

    #207399

    Purpose

    Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR

    Depositor

    Stephen Floor

    Article

    Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRFP-UtrCH

    Plasmid

    #26739

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    monomeric RFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261)

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHDR-USR-eGFP

    Plasmid

    #208830

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed eGFP but without Cas9. The selective PuroR reporter gene was designed to be repaired only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV, U6

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 HA Arf6 DN T27N

    Plasmid

    #10831

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf6 T27N (ARF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T27N. Defective in GTP binding. Dominant negati…

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-CD28-mCherry

    Plasmid

    #223593

    Purpose

    Lentiviral expression of human CD28 with mCherry tag in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD28 (CD28 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    mCherry

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1425 (pSPIN, pCDF backbone)

    Plasmid

    #160735

    Purpose

    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-LUC2CP/intron/ARE

    Plasmid

    #62858

    Purpose

    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.

    Depositor

    Gideon Dreyfuss

    Article

    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d

    Insert

    Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    insertion of chimeric intron at nueotide poclsiti…

    Promoter

    CMV

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDA_867

    Plasmid

    #231109

    Purpose

    A>G base editor

    Depositor

    John Doench, David Root

    Insert

    ABE8e-nCas9-SpG-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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