165,652 results

1. plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

   Plasmid

   #209728

   Purpose

   Expresses cytosolic high affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-SET-FLAG-HA

   Plasmid

   #24998

   Depositor

   Judy Lieberman

   Article

   Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

   Insert

   SET (SET Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG424-10X-tRNA

   Plasmid

   #70125

   Purpose

   Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Insert

   10X-tRNA block
   

   Expression

   Yeast

   Promoter

   None – SUP4 pol III promoter provided by insert

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

4. PNCA-GFP

   Plasmid

   #67789

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

   Insert

   MMLV-LTR-GFP
   

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDRM209 LTP (Luciferase tdTomato Puro)

   Plasmid

   #174723

   Purpose

   Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   tdTomato
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and T2A linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-FOXA2

   Plasmid

   #153110

   Purpose

   Expression of Flag tagged human FOXA2 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXA2 (FOXA2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ALK5 T204D

   Plasmid

   #80877

   Purpose

   mammalian expression of constitutively active ALK5 T204D

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T204D (constitutively active)

   Promoter

   CMV

   Available Since

   Aug. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDEST47-SAR1-GFP

   Plasmid

   #67409

   Purpose

   Mammalian expression of hSar1 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. PB_TRE_sg61-PDGFR_EF1a_eGFP

   Plasmid

   #186310

   Purpose

   Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg61-PDGFR
   eGFP
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0675

    Plasmid

    #141659

    Purpose

    Lentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PRDM16 (PRDM16 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX iLOV

    Plasmid

    #26587

    Depositor

    John Christie

    Article

    Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

    Insert

    iLOV
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3 WT B-Myb

    Plasmid

    #25965

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (MYBL2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nuclear Myosin I YFP

    Plasmid

    #60241

    Purpose

    Expresses nuclear isoform of myosin IC attached to YFP and an NLS

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

    Plasmid

    #118617

    Purpose

    Lentiviral expression of mTurq2 tagged PCNA

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    TK-NLS-mTurq2-PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mTurq2

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCB094 FoMV-sgLw

    Plasmid

    #234816

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbLw
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx-HTNC

    Plasmid

    #13763

    Purpose

    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

    Depositor

    Klaus Rajewsky

    Article

    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

    Insert

    His-TAT-NLS-Cre
    

    Use

    Baculovirus

    Tags

    His Tag, NLS, and TAT

    Expression

    Bacterial

    Promoter

    p10 promoter

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HA (New MCS)

    Plasmid

    #32530

    Depositor

    Christopher A Walsh

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-ARF

    Plasmid

    #89123

    Purpose

    mammalian expression of p14-hARF with N-terminal FLAG tag

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lck-mTurquoise2

    Plasmid

    #98822

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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