163,486 results

1. pcDNA3-RAB-EKARev

   Plasmid

   #118474

   Purpose

   ddRFP-based single-color biosensor for monitoring ERK activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   RAB-EKARev
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBB-XIAP

   Plasmid

   #11558

   Depositor

   Jon Ashwell

   Article

   Yang et al Science 2000 May 5;288(5467):874-7.

   Insert

   XIAP (XIAP Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmRFP-N1 human cofilin WT

   Plasmid

   #50856

   Purpose

   expresses human cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 (CFL1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. MGAT2-RpHLuorin2

   Plasmid

   #171718

   Purpose

   Cis-/medial-Golgi expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57-MCS1-P2A-MCS2-mDux

   Plasmid

   #138320

   Purpose

   Dox-inducible codon-optimized mouse Dux

   Depositor

   Yi Zhang

   Article

   Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

   Insert

   Dux (Dux Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRZ-CAS9-mCherry

   Plasmid

   #80974

   Purpose

   Cas9, mCherry expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBABE-puro HGF

   Plasmid

   #10901

   Depositor

   Bob Weinberg

   Article

   Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

   Insert

   HGF (HGF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV MP-84 mRFP

   Plasmid

   #206926

   Purpose

   The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

   Depositor

   Markus Grompe

   Article

   Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

   Insert

   mRFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MP-84

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. EMTB-mCherry

   Plasmid

   #26742

   Depositor

   William Bement

   Article

   Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Probe for microtubules

   Tags

   2x mCherry and Myc

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #161928

    Purpose

    Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

    Insert

    Surface marker and Citrine activation reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-Occludin-N-10

    Plasmid

    #55112

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Occludin (OCLN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(+)-Cyto-CaNARi

    Plasmid

    #64849

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Use

    Tags

    Cerulean, Nuclear export signal (NES), and Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP1-N1-Dsup

    Plasmid

    #90020

    Purpose

    To express GFP-tagged Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_GPHN.FingR-mKate2-IL2RGTC

    Plasmid

    #46297

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    GPHN.FingR-mKate2-IL2RGTC (GPHN Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMV306hsp

    Plasmid

    #26155

    Depositor

    Siouxsie Wiles, Brian Robertson

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    hsp60 promoter
    

    Use

    Mycobacteria integrating vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. HRE-doUnaG

    Plasmid

    #201713

    Purpose

    Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

    Depositor

    Friedemann Kiefer

    Article

    Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available Since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-EF1a-Flpo

    Plasmid

    #55637

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV2

    Insert

    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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