165,307 results

1. pPB-cT3G-cERP2-JUNB

   Plasmid

   #192907

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of JUNB

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   JUNB (JUNB Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-Z02

   Plasmid

   #120045

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   TagBFP
   

   Use

   Pcr template

   Tags

   TagBFP

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

   Plasmid

   #159129

   Purpose

   expresses NRF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   NRF2 (NFE2L2 Human)
   

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GADD45 WT-luc

   Plasmid

   #8356

   Depositor

   Michael Greenberg

   Article

   Tran et al Science 2002 Apr 19;296(5567):530-4.

   Insert

   GADD45 promoter WT (GADD45A Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. DD-Cas9 with filler sequence and Venus (EDCPV)

   Plasmid

   #90085

   Purpose

   This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH3U3-zif268 (omega)

   Plasmid

   #18046

   Depositor

   Scot Wolfe

   Article

   Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

   Insert

   zif268 binding site
   

   Expression

   Bacterial

   Mutation

   The preferred binding site for zif268 is inserted…

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSJ95

   Plasmid

   #104343

   Purpose

   Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Chen et al Science. 2017 Jan 27;355(6323). pii: 355

   Insert

   FBP1
   

   Tags

   3xFLAG

   Expression

   Yeast

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. fwYellow chromoprotein

   Plasmid

   #117841

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses fwYellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, fwYellow
   

   Use

   Synthetic Biology; Escherichia coli

   Mutation

   BioBrick sites removed

   Available Since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 9ao-SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)

   Plasmid

   #209293

   Purpose

   mitoBE expression vector, target mtND4 gene.

   Depositor

   Wensheng Wei

   Article

   Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

   Insert

   SOD2-3xHA-TALE(ND4-Left)-Nt.BspD6I(C)
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDY0209 ACTB Nicking guide +48

    Plasmid

    #179109

    Purpose

    ACTB N-term Nicking guide 1 +48 guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term Nicking guide 1 +48 guide
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-mTagBFP-CAAX

    Plasmid

    #75149

    Purpose

    Membrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mTagBFP-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E100 Hs.GRB2-nostop

    Plasmid

    #70384

    Purpose

    Gateway ORF clone of human GRB2 [NM_002086.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    GRB2 (GRB2 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAD-pHuji

    Plasmid

    #61555

    Purpose

    Red fluorescent protein based pH indicator in bacterial expression vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-FLT3-D835Y-V5/HIS

    Plasmid

    #236007

    Purpose

    expression of the D835Y kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FLT3-D835Y receptor tyrosine kinase, full length (FLT3 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    D835Y substitution

    Promoter

    CMV

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E126 Hs.MAPK1-nostop

    Plasmid

    #70410

    Purpose

    Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PMXS-NDI1

    Plasmid

    #72876

    Purpose

    expresses NDI1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

    Insert

    NDI1 (NDI1 Budding Yeast)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. T7-CMVtrans-FFLuc-polyA

    Plasmid

    #101156

    Purpose

    Template for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.

    Depositor

    Marcel Bruchez

    Article

    Canady et al J Am Chem Soc. 2015 Aug 19;137(32):10268

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Promoter

    T7 promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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