PurposeS. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||99532||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)2909
Entrez GeneTIR1 (a.k.a. AT3G62980, AtTIR1, TRANSPORT INHIBITOR RESPONSE 1)
- Promoter ADH1
/ Fusion Protein
- 9xMyc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XmaI (unknown if destroyed)
A portion of this plasmid was derived from a plasmid made byThe TIR1 gene was a kind gift from Masato Kanemaki (National Institute of Genetics, Mishima, Japan).
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Digest this vector with EcoRV for integration into TRP1.
Addgene NGS results found a single nucleotide change compared to NM_116163.4 that results in T591I within the TIR1 translation. The protein is noted by the depositing laboratory to be functional in vivo
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YIp204-PADH1-atTIR1-9myc was a gift from Helle Ulrich (Addgene plasmid # 99532 ; http://n2t.net/addgene:99532 ; RRID:Addgene_99532)