163,486 results

1. pRK-FLAG-UbxD8

   Plasmid

   #53777

   Purpose

   express FLAG-tagged human UbxD8 gene

   Depositor

   Yihong Ye

   Article

   Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

   Insert

   UbxD8 (FAF2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCLXEBR-pTF-hM3Dq

   Plasmid

   #114283

   Purpose

   TET-inducible expression of the hM3Dq protein

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   hM3Dq
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWPI-N3ICD

   Plasmid

   #185524

   Purpose

   Constitutive overexpression of human NOTCH3 intracellular domain

   Depositor

   Sean Palecek, Eric Shusta

   Article

   Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

   Insert

   NOTCH3 intracellular domain (NOTCH3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-dCas9-FLp300

   Plasmid

   #61356

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-U6+27-Tornado-F30-Squash-Broccoli

   Plasmid

   #177918

   Purpose

   Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-F30-Squash-Broccoli
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-IRES-tdTomato-FlagAkt1

   Plasmid

   #64831

   Purpose

   Lentiviral expression of murine Flag-Akt1

   Depositor

   Eva Gonzalez

   Article

   Kajno et al Biochem J. 2015 Apr 9.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PRExpress-Bxb1-hsp70pA

   Plasmid

   #158391

   Purpose

   heat shock inducible Bxb1 integrase for drosophila melanogaster

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   Bxb1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLminP_Luc2P_RE26

   Plasmid

   #90368

   Purpose

   C/EBPb - CEBPB gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CEBPb

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SHP2 WT

    Plasmid

    #8381

    Depositor

    Ben Neel

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. nesprin TSmod 25

    Plasmid

    #125555

    Purpose

    Nesprin-2G force sensor with cerulean-F25-venus TSmodule

    Depositor

    Daniel Conway

    Article

    Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

    Insert

    mini-nesprin-2G (Syne2 Mouse)
    

    Use

    Tags

    cerulean, venus

    Expression

    Mammalian

    Mutation

    TSmod changed from mTFP to cerulean, flageliform …

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEBB Flag GCN5

    Plasmid

    #74784

    Purpose

    express of GCN5 in mammalian cells

    Depositor

    Ezra Burstein

    Article

    Mao et al Genes Dev. 2009 Apr 1;23(7):849-61. doi:

    Insert

    GCN5 (KAT2A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available Since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-EGFP-Cdc42(Q61L)

    Plasmid

    #12600

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (Q61L) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIND-HA-ADH

    Plasmid

    #11991

    Depositor

    Steven Johnson

    Insert

    aldehyde dehydrogenase (ALDH1A1 Human)
    

    Use

    Ecdysone inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pJJB296

    Plasmid

    #107691

    Purpose

    35SPromoter-LwaCas13a-HSPTerminator

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pM-CBE

    Plasmid

    #196293

    Purpose

    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV M antigenome encoding CBE in place of viral GP
    

    Use

    CRISPR

    Tags

    3×flag

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEFBOS_CreIRESpuro

    Plasmid

    #183812

    Purpose

    Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

    Depositor

    Ed Stanley

    Article

    Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFN43

    Plasmid

    #89487

    Purpose

    gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

    Depositor

    Alejandro Ferrando

    Article

    Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

    Type

    Empty backbone

    Use

    Tags

    YFP-N (aa 1-154)

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p6XHis_NLS-SaCas9

    Plasmid

    #101086

    Purpose

    Express SaCas9 in bacteria with a 6xHis tag for purification

    Depositor

    Rick Tarleton

    Article

    Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

    Insert

    CRISPR-associated protein Cas9/Csn1
    

    Use

    CRISPR

    Tags

    TrxA-6xHis-STag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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