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179,813 results

Showing: 10141 - 10160 of 179813 results

  1. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Publication
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Available since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61576-plasmid-map-sequence-id-209910

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  2. pSBbi-RN

    Plasmid
    #60519
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60519-plasmid-map-sequence-id-97527

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  4. pHL-H1-ccdB-mEF1a-RiH

    Plasmid
    #60601
    Purpose
    Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.
    Depositor
    Akitsu Hotta
    Publication
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Red Fluorescent Protein
    Hygromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60601-plasmid-map-sequence-id-97111

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  8. AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60225
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA targeting LacZ. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    Oct. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60225-plasmid-map-sequence-id-96391

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  11. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid
    #55200
    Purpose
    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55200-plasmid-map-sequence-id-85587

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  12. mEmerald-mDia1-N-14

    Plasmid
    #54157
    Purpose
    Localization: Cytoskeleton, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mDia1 (DIAPH1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    54157-plasmid-map-sequence-id-475534

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  13. PA-mCherry1-C1

    Plasmid
    #54495
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54495-plasmid-map-sequence-id-218682

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  14. PA-mCherry1-N1

    Plasmid
    #54507
    Purpose
    Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  15. mCit-4aa-SNAP25B

    Plasmid
    #53235
    Purpose
    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SNAP-25B (Snap25 Rat)
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53235-plasmid-map-sequence-id-421058

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  16. mCer-4aa-VAMP2

    Plasmid
    #53233
    Purpose
    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    4aa-linker and mCerulean
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pHCAG-L2EOP

    Plasmid
    #51783
    Purpose
    The oriP/EBV nuclear antigen (EBNA)-1 elements of Epstein-Barr virus is fused in frame with the luciferase in the herpes simplex virus type-1 amplicon vector, provides prolonged transgene expression
    Depositor
    Paula Lam
    Publication
    Sia et al J Gene Med. 2010 Oct;12(10):848-58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Luciferase-2A-EBNA-1
    OriP
    Use
    Herpes simplex virus type-1 amplicon vector
    Promoter
    CAG
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    51783-plasmid-map-sequence-id-79421

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  19. pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50467
    Purpose
    Gi-coupled hM4D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has service
    AAV2 and AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50467-plasmid-map-sequence-id-199198

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Showing: 10141 - 10160 of 179813 results