PurposeBacterial expression of Amphioxus GFPa1
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60749||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth instructionsProtein can be expressed in BL21 codon+ cells. It should be grown at 37C for 4 hours followed by 20C for 6 hours.
Copy numberLow Copy
SpeciesBranchiostoma floridae (amphioxus)
Insert Size (bp)741
- Promoter T7
/ Fusion Proteins
- 6xHis (N terminal on insert)
- 6xHis (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:bfloGFPa1 was a gift from Dimitri Deheyn (Addgene plasmid # 60749 ; http://n2t.net/addgene:60749 ; RRID:Addgene_60749)
For your References section:Spectral and structural comparison between bright and dim green fluorescent proteins in Amphioxus. Bomati EK, Haley JE, Noel JP, Deheyn DD. Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469. 10.1038/srep05469 PubMed 24968921