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bfloGFPa1
(Plasmid #60749)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60749 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET24b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein can be expressed in BL21 codon+ cells. It should be grown at 37C for 4 hours followed by 20C for 6 hours.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFPa1
  • Species
    Branchiostoma floridae (amphioxus)
  • Insert Size (bp)
    741
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    bfloGFPa1 was a gift from Dimitri Deheyn (Addgene plasmid # 60749 ; http://n2t.net/addgene:60749 ; RRID:Addgene_60749)
  • For your References section:

    Spectral and structural comparison between bright and dim green fluorescent proteins in Amphioxus. Bomati EK, Haley JE, Noel JP, Deheyn DD. Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469. 10.1038/srep05469 PubMed 24968921