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172,994 results

Showing: 10181 - 10200 of 172994 results

  5. pCDH-EF1-FHC-POLQ-K121M

    Plasmid
    #64877
    Purpose
    lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain)
    Depositor
    Richard Wood
    Article
    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.
    Insert
    POLQ (POLQ Human)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    K121M, a mutation in the conserved ATP-binding si…
    Promoter
    EF1alpha
    Available Since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    64877-plasmid-map-sequence-id-163756

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  8. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid
    #74315
    Purpose
    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish
    Depositor
    Martin Meyer, Stephen Smith
    Article
    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub
    Inserts
    zebrafish PSD95
    DsRedExpress
    Tags
    EGFP
    Mutation
    Y599H and I793S (please see depositor comment bel…
    Available Since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. mEGFP-pBAD

    Plasmid
    #54622
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    6xHis and mEGFP
    Expression
    Bacterial
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54622-plasmid-map-sequence-id-257491

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  11. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid
    #99690
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Sept. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    99690-plasmid-map-sequence-id-194510

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  14. pAAV-hSyn-DIO-EGFP

    Plasmid
    #50457
    Purpose
    Double floxed EGFP under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    N/A
    Promoter
    human Synapsin 1
    Available Since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50457-plasmid-map-sequence-id-162265

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  17. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid
    #158135
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-Gal4VP64
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1α
    Available Since
    Oct. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158135-plasmid-map-sequence-id-309380

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  18. pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

    Plasmid
    #128450
    Purpose
    lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128450-plasmid-map-sequence-id-256464

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Showing: 10181 - 10200 of 172994 results