165,652 results

1. pLKO.1P POLA1_1

   Plasmid

   #160789

   Purpose

   Suppress POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shPOLA1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK462 (Hygro-P2A-mAB)

   Plasmid

   #214388

   Purpose

   mAID-BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   Hygro-P2A-mAID-BromoTag
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. R5 EMCV

   Plasmid

   #51733

   Purpose

   Dicistronic vector with EMCV IRES

   Depositor

   Vincent Mauro

   Article

   Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

   Insert

   EMCV IRES
   

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. RhoG

   Plasmid

   #55694

   Purpose

   expression of mouse RhoG

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RhoG (Rhog Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

5. EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

   Plasmid

   #191149

   Purpose

   Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587:Cre
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL025_HA-rb_IgG_HC_15F11

   Plasmid

   #193622

   Purpose

   HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti_HA_15F11 rabbit heavy chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pDL026_HA-rb_IgG_LC_15F11

   Plasmid

   #193623

   Purpose

   HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

   Depositor

   Jennifer DeLuca

   Article

   DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

   Insert

   Anti-HA_15F11 rabbit light chain
   

   Tags

   signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCBL101gg

   Plasmid

   #199735

   Purpose

   Golden Gate recipient vector for transgene overexpression

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   sacB
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTfR-mTurquoise2

   Plasmid

   #112953

   Purpose

   In vivo visualization of Transferrin receptors (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Insert

   pTfR
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. GG-EBNA-OSK2M2L1-PP

    Plasmid

    #102902

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OSK2M2L1-gRNAs-PGK-puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-hNox4

    Plasmid

    #69352

    Purpose

    expresses human Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    hNOX4 (NOX4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-AIF

    Plasmid

    #16182

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Apoptosis-inducing factor (AIFM1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPtPBR1

    Plasmid

    #80388

    Purpose

    Episome vector for Phaeodactylum tricornutum (pBR322-based)

    Depositor

    Philip Weyman

    Type

    Empty backbone

    Use

    Synthetic Biology; ; episomal vector for phaeodac…

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFcpB-DEST

    Plasmid

    #90098

    Purpose

    Gateway destination vector featuring FcpB promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

    Depositor

    Philip Weyman

    Article

    Bielinski et al PeerJ Preprints. 2017 5:e2907v1

    Type

    Empty backbone

    Use

    Destination vector for gateway expression

    Promoter

    FcpB

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pES001 MBP-GSK3β-HA-His

    Plasmid

    #154010

    Purpose

    Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GSK3B (GSK3B Human)
    

    Tags

    HA tag, His6, and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1 Lifeact-EGFP-2XNLS

    Plasmid

    #58467

    Purpose

    Expresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

    Inserts

    Lifeact
    2X NLS with 2X stop codons
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_NXF1_TAP-C

    Plasmid

    #110038

    Purpose

    Protein expression and purification of NXF1_TAP-C

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NXF1_TAP-C (NXF1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. RP4-8

    Plasmid

    #79813

    Purpose

    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Gentamicin resistance gene
    

    Use

    Helper bhr plasmid for mobilizing other plasmids …

    Promoter

    Own promoter

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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