167,150 results
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Plasmid#62205PurposeAn empty sgRNA expression vector for expression of sgRNA for Sp Cas9DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6Available SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
TagsHAExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
TP53-KO_gRNA_2
Plasmid#195131PurposeTP53-targeting gRNA in the LRG2.1 backboneDepositorInsertTP53 KO gRNA 2 (TP53 Human)
ExpressionMammalianAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
TNPO1-shRNA
Plasmid#86082PurposeA lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.DepositorAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-pknG
Plasmid#102814PurposeExpression of PknG in E.coliDepositorInsertpknG
TagsHisExpressionBacterialPromoterT7Available SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NLS
Plasmid#118937PurposeUsed to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
ExpressionPlantAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF0822
Plasmid#142230PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRDA_479
Plasmid#179099PurposeBE Cas expressionDepositorInsertABE8e (SpG)
UseCRISPR and LentiviralAvailable SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2078
Plasmid#141910PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMET17p-CRU313
Plasmid#131163PurposeFor ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutantDepositorTypeEmpty backboneTagsCRUExpressionYeastAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage-cmv-cfp-24xms2
Plasmid#40651DepositorInsertCFP-24xMBS
UseLentiviralTagsCFPExpressionMammalianPromoterCMVAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)
Plasmid#175574PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB2B
Plasmid#195284PurposeBicistronic HSV-TK and Puromycin resistance for positive/negative selectionDepositorInsertHSV-TK 2A Puro
ExpressionMammalianPromoterPGKAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GIPR-DuET
Plasmid#213252PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2Apuro-TRE-synPCB2.0
Plasmid#139481PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
UseTol2 transposon donor vectorExpressionMammalianPromoterTet responsive elementAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.TRC1.shmNsd1.1, puro
Plasmid#114446PurposeLentiviral vector for expression of shRNA sequence targeting mouse Nsd1DepositorInsertshNsd1.1 (Nsd1 Mouse)
UseLentiviral and RNAiAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIN4-EF1a-ETV2-IRES-Puro
Plasmid#61061Purposelentiviral vector for ETV2DepositorAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P85
Plasmid#40219DepositorAvailable SinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only