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165,567 results

Showing: 10201 - 10220 of 165567 results

  2. AICSDP-43: GJA1-mEGFP

    Plasmid
    #109120
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1
    Depositor
    Allen Institute for Cell Science
    Insert
    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109120-plasmid-map-sequence-id-209766

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  3. pLV-REPORT(EF1a)

    Plasmid
    #172326
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172326-plasmid-map-sequence-id-354701

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  6. pENTR221-BNT162b2ΔUTR

    Plasmid
    #171214
    Purpose
    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.
    Depositor
    Liang Wei Wang
    Article
    Low et al medRxiv 2021.04.27.21256151
    Insert
    BNT162b2ΔUTR
    Use
    Gateway cloning
    Mutation
    Kozak sequence changed from CCCGCCACC to GGCTGCAC…
    Available Since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171214-plasmid-map-sequence-id-336476

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  10. pGEX6P1-3xMBT

    Plasmid
    #46987
    Purpose
    Inducible expression of 3xMBT from L3MBTL1 as a GST fusion protein in E.coli
    Depositor
    Or Gozani
    Article
    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:
    Insert
    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    Tags
    GST and PreScission cleavage site
    Expression
    Bacterial
    Mutation
    contains amino acids 190–530 of accession NP_0562…
    Promoter
    tac
    Available Since
    Sept. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    46987-plasmid-map-sequence-id-66939

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  11. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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  12. IgK-IFN-luc

    Plasmid
    #14886
    Purpose
    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression
    Depositor
    David Baltimore
    Article
    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.
    Insert
    IgK-IFN (IFNB1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Available Since
    May 1, 2007
    Availability
    Academic Institutions and Nonprofits only
    14886-plasmid-map-sequence-id-238489

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  18. pSCAR_Cas9-hygro_GFP

    Plasmid
    #162075
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-hygromycin B phosphotransferase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available Since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162075-plasmid-map-sequence-id-325304

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  20. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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Showing: 10201 - 10220 of 165567 results