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Stem cells. m6A mRNA methylation facilitates resolution of naive pluripotency toward differentiation.

Geula S, Moshitch-Moshkovitz S, Dominissini D, Mansour AA, Kol N, Salmon-Divon M, Hershkovitz V, Peer E, Mor N, Manor YS, Ben-Haim MS, Eyal E, Yunger S, Pinto Y, Jaitin DA, Viukov S, Rais Y, Krupalnik V, Chomsky E, Zerbib M, Maza I, Rechavi Y, Massarwa R, Hanna S, Amit I, Levanon EY, Amariglio N, Stern-Ginossar N, Novershtern N, Rechavi G, Hanna JH
Science. 2015 Feb 27;347(6225):1002-6. doi: 10.1126/science.1261417. Epub 2015 Jan 1. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
61514px335 Mettl14 sgRNA #2encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)
61515px335 Mettl14 sgRNA #1encodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)
61516pBRPyCAG-Mettl3-dsRed-IRES-puroConstitutive expression of murine wild-type Mettl3

Antibodies from Article