PurposeExpresses monomeric streptavidin (mSA) in bacterial cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80706||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5300
- Total vector size (bp) 5660
Modifications to backboneHomemade vector derived from pET-24-a+, engineered to incorporate after the start codon a decahistidine tag immediately followed by a Tobacco Etch Virus cleavage site (-ENLYFQS-).
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameMonomeric streptavidin
Alt namestreptavidin rhizavidin hybrid
Insert Size (bp)360
- Promoter T7
/ Fusion Proteins
- 10xHis tag (N terminal on backbone)
- TEV site (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 promoter, forward primer
- 3′ sequencing primer T7 terminator, reverse primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET-IG-mSA was a gift from Matthieu Sainlos (Addgene plasmid # 80706 ; http://n2t.net/addgene:80706 ; RRID:Addgene_80706)
For your References section:Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin. Chamma I, Letellier M, Butler C, Tessier B, Lim KH, Gauthereau I, Choquet D, Sibarita JB, Park S, Sainlos M, Thoumine O. Nat Commun. 2016 Mar 16;7:10773. doi: 10.1038/ncomms10773. 10.1038/ncomms10773 PubMed 26979420