pET-IG-mSA
(Plasmid #80706)

• Purpose: Expresses monomeric streptavidin (mSA) in bacterial cells
• Depositing Lab: Matthieu Sainlos
• Publication: Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10.1038/ncomms10773.

Backbone

• Vector backbone: pET-IG
• Backbone manufacturer: Homemade
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 5660
• Modifications to backbone: Homemade vector derived from pET-24-a+, engineered to incorporate after the start codon a decahistidine tag immediately followed by a Tobacco Etch Virus cleavage site (-ENLYFQS-).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Monomeric streptavidin
• Alt name: mSA
• Alt name: streptavidin rhizavidin hybrid
• Species: Synthetic
• Insert Size (bp): 360
• Promoter: T7
• Tags / Fusion Proteins:
  • 10xHis tag (N terminal on backbone)
  • TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter, forward primer
• 3′ sequencing primer: T7 terminator, reverse primer

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry