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pET-IG-mSA
(Plasmid #80706)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80706 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-IG
  • Backbone manufacturer
    Homemade
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 5660
  • Modifications to backbone
    Homemade vector derived from pET-24-a+, engineered to incorporate after the start codon a decahistidine tag immediately followed by a Tobacco Etch Virus cleavage site (-ENLYFQS-).
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Monomeric streptavidin
  • Alt name
    mSA
  • Alt name
    streptavidin rhizavidin hybrid
  • Species
    Synthetic
  • Insert Size (bp)
    360
  • Promoter T7
  • Tags / Fusion Proteins
    • 10xHis tag (N terminal on backbone)
    • TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter, forward primer
  • 3′ sequencing primer T7 terminator, reverse primer
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-IG-mSA was a gift from Matthieu Sainlos (Addgene plasmid # 80706 ; http://n2t.net/addgene:80706 ; RRID:Addgene_80706)
  • For your References section:

    Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin. Chamma I, Letellier M, Butler C, Tessier B, Lim KH, Gauthereau I, Choquet D, Sibarita JB, Park S, Sainlos M, Thoumine O. Nat Commun. 2016 Mar 16;7:10773. doi: 10.1038/ncomms10773. 10.1038/ncomms10773 PubMed 26979420