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MEF2C Phosphorylation Is Required for Chemotherapy Resistance in Acute Myeloid Leukemia.
Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA, Armstrong SA, Kentsis A
Cancer Discov. 2018 Apr;8(4):478-497. doi: 10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
89715MSCV-IRES-Tomato MEF2CRetroviral gene expression vector for MEF2C expression
89716MSCV-IRES-Tomato MEF2C S222ARetroviral gene expression vector for MEF2C-S222A expression
89717pINDUCER20 MEF2CLentiviral gene expression vector for doxycycline inducible MEF2C expression
89718pINDUCER20 MEF2C S222ALentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression
107229MSCV-IRES_TomatoTomato expressing plasmid
107231MSCV-IRES_Tomato MEF2C S222DhMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis
107232pLT3REVIR_MARK3 1122shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one
107233pLT3REVIR_MARK3 2573shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one
107234pINDUCER20 MEF2C S222DMEF2C phosphomimetic mutant serine to aspartic acid
107235pLX304-MARK3Lentiviral gene expression vector for phospho-MARK3 expression
107236pLX304-MARK3_T211ALentiviral gene expression vector for phospho-MARK3_T221A expression
107238pRecLV105A lentiviral vector for expression in mammalian cells
107239pRecLV105 MEF2C S222AA lentiviral vector for expression in mammalian cells encoding MEF2C-S222A insert
107240pRecLV105 MEF2CA lentiviral vector for expression in mammalian cells encoding MEF2C insert

Antibodies from Article