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Nano-lantern/pcDNA3

Plasmid

#51970

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRXRα

Plasmid

#135400

Purpose

Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using CMV promoter

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Human retinoid X receptor- alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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GSK3beta (1015)

Plasmid

#49491

Purpose

expresses human Gsk3beta

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta (GSK3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Drp1K38A

Plasmid

#45161

Depositor

Alexander van der Bliek, Richard Youle

Article

Smirnova et al J Cell Biol. 1998 Oct 19;143(2):351-8.

Insert

Drp1 (DNM1L Human)

Use

Tags

HA (AYPYDVPDY)

Expression

Mammalian

Mutation

K38A

Promoter

CMV

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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TOP-GFP.mC

Plasmid

#35491

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xTOP-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7xTCF/LEF

Available since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pRC-CMV-Rev1b

Plasmid

#204153

Purpose

Lentivirus helper plasmid coding for Rev

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

Rev

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLY088_AAV_EFS-BCMA-41BBz-PRODH2

Plasmid

#192191

Purpose

BCMA CAR AAV vector PRODH2 KI (pLY088)

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

BCMA CAR AAV vector PRODH2 KI (pLY088) (TNFRSF17 Synthetic)

Use

AAV; Mammalian expression

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-AAK1

Plasmid

#23844

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AAK1 (AAK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)

Plasmid

#163503

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Boaz Levi, The Allen Institute for Brain Science

Article

Mich et al Cell Rep. 2021 Mar 30;34(13):108754. doi

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

MinRho

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-PTEN

Plasmid

#13039

Depositor

Alonzo Ross

Article

Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

Insert

PTEN (PTEN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) anti-Her2 (FRP5) scFv-Fc

Plasmid

#183249

Purpose

Soluble expression of anti-human Her2 scFvFc (clone FRP5). Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

Anti-Her2 scFvFc (FRP5)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRubiC-T2A-Cas9

Plasmid

#75347

Purpose

Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

Depositor

Bryan Luikart

Article

Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

Insert

Cas9

Use

CRISPR and Retroviral

Tags

mCherry (via t2a)

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC2A4

Plasmid

#132102

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC2A4 (SLC2A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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Lenti-pNFkB::Cre-mRuby3

Plasmid

#164137

Purpose

Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

NFkB promoter-Cre-T2A-mRuby3

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Tags

Expression

Mammalian

Mutation

Promoter

NFkB synthetic promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-beta-HA-BRCA2

Plasmid

#133553

Purpose

Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

Depositor

David Livingston

Article

Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Insert

BRCA2 (BRCA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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YAP1 (S6A) - V5 in pLX304

Plasmid

#42562

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CD

Plasmid

#174190

Purpose

Expresses PIK3CD in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CD (PIK3CD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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R777-E137 Hs.MTOR

Plasmid

#70421

Purpose

Gateway ORF clone of human MTOR [NM_004958.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

MTOR (MTOR Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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HA-SOCS1

Plasmid

#174571

Purpose

Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

Depositor

Mathew Garnett

Article

Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

Insert

HA-SOCS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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