pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
(Plasmid #128424)

• Purpose: Human Codon Optimized dCas9-DNMT3A3L
• Depositing Lab: Bradley Bernstein
• Publication: Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi: 10.1038/s41467-019-12166-w.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 12000
• Modifications to backbone: pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP Human codon optimized dCas9-Dnmt3a-Dnmt3l
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
• Species: Synthetic; Streptococcus pyogenes Type II
• Insert Size (bp): 6639
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GAGCGGATAACAATTTCACACAGG
• 3′ sequencing primer: CGCAAATGGGCGGTAGGCGTG

Resource Information

• Supplemental Documents:
  • pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP(1).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry