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pXJ219-pAAV-PB3'IR-U6-gRNA-EF1a-mtagBFP2-KASH-PB5'IR

Plasmid

#210525

Purpose

AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mtagBFP-KASH
U6-gRNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJeM1

Plasmid

#135088

Purpose

rhamnose-inducible expression vector for replication in E. coli and P. putida

Depositor

Josef Altenbuchner

Article

Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

Insert

rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_C4341S

Plasmid

#187156

Purpose

mammalian cell expression FLAG-HUWE C4341S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

C4341S

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Vac 14 Full Length

Plasmid

#47418

Depositor

Peter McPherson

Article

Lemaire et al FEBS Lett. 2006 Dec 22;580(30):6948-54.

Insert

Vac14 (VAC14 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCW57.1 blast tBID-flag

Plasmid

#211533

Purpose

Expresses truncated BID upon doxycycline addition to trigger apoptosis

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

tBID-flag (BID Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Truncated (CASP8) form (p15)

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-Ezh2

Plasmid

#24926

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 27, 2010

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18

Plasmid

#164436

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA

Plasmid

#168305

Purpose

mediated knock-in of sgRNA precursor

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

SaKKH-EGFP-U6-sgRNA

Use

AAV

Expression

Mammalian

Promoter

EF1alpha, CBh

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Tags

His

Expression

Bacterial

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCre-MBP1-WPRE

Plasmid

#193917

Purpose

Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

CCre-MBP1

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCre-MBP6-WPRE

Plasmid

#193916

Purpose

Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

NCre-MBP6

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FLAG-CHK1

Plasmid

#86875

Purpose

To express CHK1 with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

CHEK1 (CHEK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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hFerritinH

Plasmid

#122652

Purpose

Expression of hFerritinH in bacterial cells

Depositor

Trevor Douglas

Insert

ferritin heavy chain 1 (FTH1 Human)

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT3.0

Plasmid

#208717

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Has Service

AAV2 and AAV8

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pPSU100

Plasmid

#173835

Purpose

Expresses 100 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B7

Plasmid

#135509

Purpose

Mammalian expression of myc-tagged HLA-B*07:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*07:02 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A WT

Plasmid

#123089

Purpose

Expresses 3xFLAG-LC3A wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

Plasmid

#149722

Purpose

Lentiviral expression of crRNA with GFP and a neomycin resistance gene

Depositor

Junwei Shi

Article

Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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