165,255 results

1. VP12

   Plasmid

   #72247

   Purpose

   Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A and Q926A

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Rtn3S-GFP

   Plasmid

   #169035

   Purpose

   Express human Rtn3S in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rtn3S (RTN3 Human)
   

   Tags

   acGFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHCMV-SS-antiFLAGscFv-TM

   Plasmid

   #198303

   Purpose

   Displays anti-FLAG scFv from the surface of mammalian cells

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   pHCMV-SS-antiFLAGscFv-TM
   

   Expression

   Mammalian

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL5.0 E-cadherin shRNA/Ecad-tdTomato

   Plasmid

   #101278

   Purpose

   Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   E-cadherin
   

   Use

   Lentiviral

   Tags

   tdtomato

   Expression

   Mammalian

   Available Since

   Jan. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-C1ab-Prkd1

   Plasmid

   #139314

   Purpose

   Diacylglycerol biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
   

   Expression

   Mammalian

   Mutation

   T208A, N313D

   Available Since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-VEGFR1-V5/HIS

   Plasmid

   #205616

   Purpose

   Expression of human VEGFR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   VEGFR1 receptor tyrosine kinase (FLT1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. px459V2.0_Conc2

   Plasmid

   #134451

   Purpose

   Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + Puro

   Depositor

   Daniel Stange

   Article

   Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. iRFP670-KASH2

   Plasmid

   #187021

   Purpose

   Inducible disruption of LINC Complex

   Depositor

   Jan Lammerding

   Insert

   KASH2
   

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-MCS

   Plasmid

   #185594

   Purpose

   Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH2.32_LV-mCherry-MS2x8-BCS

    Plasmid

    #205539

    Purpose

    Express mCherry-MS2x8 with barcode

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX-RCC1-HA

    Plasmid

    #106938

    Purpose

    Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

    Insert

    human RCC1-HA (RCC1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-FH-AGO2-WT

    Plasmid

    #91978

    Purpose

    Expresses FLAG-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HIP-flash (HOP-flash mutant)

    Plasmid

    #83466

    Purpose

    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLY100-RetroEFS-HER2-28BBz-WPRE

    Plasmid

    #192201

    Purpose

    Retro-HER2CAR

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Mutation

    NA

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-NLS-GFP

    Plasmid

    #104061

    Purpose

    AAV expression of nuclear localized GFP with SV40 NLS

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-BICD2

    Plasmid

    #164626

    Purpose

    Expresses BICD2 in mammalian cells with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    BICD2 (BICD2 Human)
    

    Tags

    Cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-M-Flag-BAP1-C91G

    Plasmid

    #125845

    Purpose

    express mutant BAP1-C91G

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    BAP1 (BRCA1 associated protein 1) (BAP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    C91G

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1(+)-NEMOm

    Plasmid

    #189930

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-gTEMP/pcDNA3

    Plasmid

    #188447

    Purpose

    B-gTEMP for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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