165,255 results

1. AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787)

   Plasmid

   #191726

   Purpose

   AI plasmid ALIAS: AiP12787. Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   SYFP2
   

   Use

   AAV

   Mutation

   none

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-iLACCO1.2

   Plasmid

   #218823

   Purpose

   Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1.2
   

   Expression

   Mammalian

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

   Plasmid

   #60224

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   KrasG12D HDR donor (Kras Mouse)
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Promoter

   EFS, No promoter, and U6

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-GAL4

   Plasmid

   #24345

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   GAL4
   

   Expression

   Mammalian

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

   Plasmid

   #229791

   Purpose

   Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

   Plasmid

   #159816

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-CAG-empty-pgk-hph

   Plasmid

   #48753

   Purpose

   PiggyBac backbone, CAG promoter, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Type

   Empty backbone

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pDK521

   Plasmid

   #134204

   Purpose

   Baculovirus expresion of Astrin, LC8, and SKAP

   Depositor

   Iain Cheeseman

   Article

   Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

   Insert

   Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
   

   Expression

   Insect

   Available Since

   Feb. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 791 p3E-2A-pCS2MCSpA v2

   Plasmid

   #195973

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-multiple cloning site
   

   Use

   Other

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-VHL-pRc/CMV

    Plasmid

    #19999

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-flag-GFP-iCAM-1

    Plasmid

    #205186

    Purpose

    This vector encodes of a synCAM (iCAM1) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TadCBE-Cas9NG

    Plasmid

    #225093

    Purpose

    Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

    Insert

    TadCBE-Cas9NG
    

    Use

    CRISPR

    Tags

    8xHis

    Expression

    Bacterial

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-sgRNA-Cre-GpNluc-sgControl

    Plasmid

    #208383

    Purpose

    This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgControl (TP53BP1 intron) (Trp53bp1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pS-sg:GFP

    Plasmid

    #196296

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB513

    Plasmid

    #74855

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid

    #71294

    Purpose

    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    GNSTM glycosylation motif, HA, Lamp2 signal pepti…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRetroX-TRE3G_NINJ1

    Plasmid

    #201181

    Purpose

    Dox-inducible expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Promoter

    TRE3G

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMDC32-HPB

    Plasmid

    #32078

    Depositor

    Fumi Katagiri

    Article

    Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

    Type

    Empty backbone

    Use

    Gateway destination binary vector

    Tags

    HA-Prescission-Biotin

    Promoter

    35S promoter

    Available Since

    Dec. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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