|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||30179||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerRamakrishnan et al., 2000
- Backbone size w/o insert (bp) 4981
Vector typeMycobacteria expression
Growth in Bacteria
Gene/Insert nameMycobacterium Strong Promoter (MSP)
Insert Size (bp)500
/ Fusion Protein
- mKate2 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer pFPV27-Fwd: GAATCGGTGGTTGTGGTGAT
- 3′ sequencing primer Kan-Rev (Common Sequencing Primers)
This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance. The GFP was then replaced with mKate2. The mKate2 ORF is present at bp# 105-803.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTEC20 was a gift from Lalita Ramakrishnan (Addgene plasmid # 30179 ; http://n2t.net/addgene:30179 ; RRID:Addgene_30179)
For your References section:Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish. Takaki K, Davis JM, Winglee K, Ramakrishnan L. Nat Protoc. 2013 Jun;8(6):1114-24. doi: 10.1038/nprot.2013.068. Epub 2013 May 16. 10.1038/nprot.2013.068 PubMed 23680983