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(Plasmid #69007)


Item Catalog # Description Quantity Price (USD)
Plasmid 69007 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4733
  • Total vector size (bp) 5893
  • Modifications to backbone
    EGFP replaced with sfGFP and the V207 in sfGFP mutagenized to K with PCR cloning to monomerize.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gja1 (a.k.a. Cx43, Cxnk1)
  • Promoter cmv
  • Tag / Fusion Protein
    • monomerized superfolder GFP (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV forward primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cx43-msfGFP was a gift from David Spray (Addgene plasmid # 69007 ; ; RRID:Addgene_69007)
  • For your References section:

    Connexin Type and Fluorescent Protein-fusion Tag Determine Structural Stability of Gap Junction Plaques. Stout RF Jr, Snapp EL, Spray DC. J Biol Chem. 2015 Aug 11. pii: jbc.M115.659979. 10.1074/jbc.M115.659979 PubMed 26265468