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p189
(Plasmid #65207)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 65207 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM5Z(+)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3001
  • Total vector size (bp) 4604
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    1632
  • Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG(58)-->TAG)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To be used in conjunction with pGEM5Z(+)-EGFP (p111): www.addgene.org/65206. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p189 was a gift from LuZhe Sun (Addgene plasmid # 65207 ; http://n2t.net/addgene:65207 ; RRID:Addgene_65207)
  • For your References section:

    Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivo mismatch repair activity assay. Zhou B, Huang C, Yang J, Lu J, Dong Q, Sun LZ. Anal Biochem. 2009 May 1;388(1):167-9. doi: 10.1016/j.ab.2009.02.020. Epub 2009 Feb 25. 10.1016/j.ab.2009.02.020 PubMed 19248754