166,276 results

1. pENTR223.1_mmDdx58 (closed)

   Plasmid

   #160107

   Purpose

   Expresses murine Ddx58 in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Ddx58 (Rigi Mouse)
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM1s3AsG

   Plasmid

   #137921

   Purpose

   E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and constitutive GFP expression

   Depositor

   Neel Joshi

   Article

   Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

   Insert

   Superfolder GFP
   

   Use

   Synthetic Biology; For use with e. coli nissle in…

   Expression

   Bacterial

   Promoter

   J23101

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX-sgRNA

   Plasmid

   #50662

   Purpose

   Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

   Insert

   sgAAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. slc1a3b:myrGFP-P2A-H2AmCherry

   Plasmid

   #170208

   Purpose

   Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGFP-P2A-H2AmCherry

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP28

   Plasmid

   #194707

   Purpose

   Linker::3xFlag::mScarlet::HAtag::Linker

   Depositor

   Alexandre Paix

   Article

   Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

   Insert

   Linker::3xFlag::mScarlet::HAtag::Linker
   

   Use

   Synthetic Biology

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

   Plasmid

   #169449

   Purpose

   expression of TRF1-mcherrry and sgRNA targeting telomeres

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   mCherry-TRF1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. GSN-bio-His

   Plasmid

   #52067

   Purpose

   Expresses full-length Gelsolin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   GSN (GSN Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-GFP-MKLP1

   Plasmid

   #140566

   Purpose

   Expresses GFP tagged MLKP1

   Depositor

   Heidi Hehnly

   Article

   Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

   Insert

   MKLP1 (KIF23 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-CMV-hIL7-IRES-ZsGreen-W

    Plasmid

    #26532

    Depositor

    David Baltimore

    Insert

    IL7 (IL7 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mAIM2-Flag

    Plasmid

    #51537

    Purpose

    Express Flag-tagged mAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-CEACAM1-COMP5AP-AviTag-9xHis

    Plasmid

    #157390

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CEACAM1 (CEACAM1 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTM

    Plasmid

    #162783

    Purpose

    Protein expression in CHO cells - hTPA leader and C-terminal c-myc and 10xhis tags are coded

    Depositor

    Ivan Vorobiev

    Article

    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi

    Type

    Empty backbone

    Tags

    10xHis, c-myc, and hTPA leader

    Expression

    Mammalian

    Promoter

    CHO Elongation factor 1A

    Available Since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. eMagAF-EGFP-PM

    Plasmid

    #162247

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagAF-EGFP-CAAX
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-GfABC1D-NLS-GFP-WPRE-synpA-L1-R2

    Plasmid

    #203541

    Purpose

    gRNA expression in astrocytes

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    NLS-GFP, 2x U6-RNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBTK562 (pDS-GFP)

    Plasmid

    #183129

    Purpose

    Control plasmid that expresses off-target dsRNA

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al Science. 2020 Jan 31;367(6477):573-576.

    Insert

    GFPmut3 (no RBS)
    

    Use

    Synthetic Biology

    Available Since

    July 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDONR221_SLC9A1

    Plasmid

    #132210

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC9A1 (SLC9A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBdest-GST-PI3Kc1

    Plasmid

    #187992

    Purpose

    Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

    Depositor

    Sascha Martens

    Inserts

    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    

    Tags

    GST

    Expression

    Bacterial and Insect

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS mPrx1 Cre (CT#644)

    Plasmid

    #13962

    Depositor

    Cliff Tabin

    Article

    Logan et al Genesis. 2002 Jun . 33(2):77-80.

    Insert

    Prx1 Cre (Prrx1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only