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pBHRSF184
(Plasmid #89482)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89482 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHYRSF53
  • Backbone size w/o insert (bp) 4540
  • Total vector size (bp) 5245
  • Modifications to backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Asparaginyl endopeptidase from Oldenlandia affinis
  • Alt name
    OaAEP1
  • Species
    Synthetic
  • Insert Size (bp)
    1353
  • Promoter T7
  • Tag / Fusion Protein
    • Smt3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHRSF184 was a gift from Hideo Iwai (Addgene plasmid # 89482 ; http://n2t.net/addgene:89482 ; RRID:Addgene_89482)
  • For your References section:

    Segmental isotopic labeling of a single-domain globular protein without any refolding step by an asparaginyl endopeptidase. Mikula KM, Tascon I, Tommila JJ, Iwai H. FEBS Lett. 2017 Mar 30. doi: 10.1002/1873-3468.12640. 10.1002/1873-3468.12640 PubMed 28369872