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pQE30-butelase1
(Plasmid #115430)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 115430 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    QIAGEN
  • Backbone size w/o insert (bp) 3450
  • Total vector size (bp) 4831

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    butelase 1
  • Alt name
    CtAEP1
  • Species
    Synthetic
  • Insert Size (bp)
    1400
  • Promoter T5
  • Tag / Fusion Protein
    • 6-His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CCCGAAAAGTGCCACCTG
  • 3′ sequencing primer GTTCTGAGGTCATTACTGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Is a synthetic gene, codon optimised for E. coli made upon request by GENEART (14AB5UDP)
  • Terms and Licenses

Depositor Comments

Expression and purification details in bioRxiv preprint “The macrocyclizing protease butelase 1 remains auto-catalytic and reveals the structural basis for ligase activity” by Amy M James, Joel Haywood, Julie Leroux, Katarzyna Ignasiak, Alysha G Elliott, Jason W Schmidberger, Mark F Fisher, Samuel G Nonis, Ricarda Fenske, Charles S Bond, Joshua S Mylne
doi: https://doi.org/10.1101/380295 . Please visit https://www.biorxiv.org/content/early/2018/07/30/380295 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-butelase1 was a gift from Joshua Mylne (Addgene plasmid # 115430 ; http://n2t.net/addgene:115430 ; RRID:Addgene_115430)
  • For your References section:

    The macrocyclizing protease butelase 1 remains auto-catalytic and reveals the structural basis for ligase activity. James AM, Haywood J, Leroux J, Ignasiak K, Elliott AG, Schmidberger JW, Fisher MF, Nonis SG, Fenske R, Bond CS, Mylne JS. Plant J. 2019 Feb 21. doi: 10.1111/tpj.14293. 10.1111/tpj.14293 PubMed 30790358