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A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina.
Wang S, Sengel C, Emerson MM, Cepko CL
Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
69992CRISPRmTmG2The CRISPR construct targets near the LoxP sites in Rosa-pCA-loxP-mTdtomato-loxP-mEGFP mice.
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73977CAG-EGFP-LacZshRNALacZshRNA (mir155 backbone) was inserted into the 3'UTR of EGFP.
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73978CAG-mCherry-LacZshRNALacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.
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73979CAG-mCherry-Otx2shRNA1Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.
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73980CAG-mCherry-Otx2shRNA2Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.
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73981CAG-EGFP-Otx2shRNA1Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.
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73982CAG-EGFP-Otx2shRNA2Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.
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73983CAG-mCherry-RORbshRNA1RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.
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73984CAG-mCherry-RORbshRNA2RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.
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73985CAG-EGFP-RORbshRNA1RORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.
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73986CAG-EGFP-RORbshRNA2RORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.
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73987CAG-mCherry-CrxshRNACrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.
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73988B108-GFP-IRES-APB108 enhancer of Blimp1 gene drives EGFP expression.
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73989B108-CreB108 enhancer of Blimp1 gene drives Cre expression.
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73990B108-Cre2B108 enhancer of Blimp1 gene drives IRES-Cre expression.
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73991Stagia3-CreCre in Stagia3 backbone; vector-Cre
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73992Chx10BP-LCL-EGFPCre-dependent reporter plasmid for bipolar cells in the retina
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73993B108-Otx2-IRES-CreB108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.
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73995CAG-Blimp1Plasmid expressing mouse Blimp1
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73996CAG-Otx2-IRES-GFPd2Plasmid expressing Otx2(isoform b)-IRES-GFPd2
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73997CAG-Crx-IRES-GFPd2Plasmid expressing Crx-IRES- GFPd2
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73998CAG-RORb2-IRES-GFPd2Plasmid expressing RORb2-IRES-GFPd2
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73999CAG-RORb2Overexpression of RORb2
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74005B51gRNA for B108
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74006B53gRNA for B108
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74007B31gRNA for B108
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74008B33gRNA for B108
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