CAG-RORb2
(Plasmid #73999)

• Purpose: Overexpression of RORb2
• Depositing Lab: Connie Cepko
• Publication: Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.

Backbone

• Vector backbone: pCAG-GFP
• Backbone manufacturer: addgene plasmid #11150
• Backbone size w/o insert (bp): 4792
• Total vector size (bp): 6205
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RORb2 ORF
• Alt name: RAR-related orphan receptor beta
• Species: M. musculus (mouse)
• Insert Size (bp): 1413
• GenBank ID: NM_146095.4 NM_146095.4
• Entrez Gene: Rorb (a.k.a. Nr1f2, RZR-beta, RZRB, Rorbeta, hstp)
• Promoter: CAG
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: gttcggcttctggcgtgt

Resource Information

• Supplemental Documents:
  • CAG-RORb2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry