PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73982||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene (Plasmid #11150)
- Backbone size w/o insert (bp) 5552
- Total vector size (bp) 5685
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
SpeciesM. musculus (mouse)
- Promoter CAG
- Cloning method Unknown
- 5′ sequencing primer gttcggcttctggcgtgt
- 3′ sequencing primer TTTTGGCAGAGGGAAAAAGA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CAG-EGFP-Otx2shRNA2 was a gift from Connie Cepko (Addgene plasmid # 73982 ; http://n2t.net/addgene:73982 ; RRID:Addgene_73982)
For your References section:A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Wang S, Sengel C, Emerson MM, Cepko CL. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21. 10.1016/j.devcel.2014.07.018 PubMed 25155555